Subunit composition and function of GABA(A) receptors of rat spermatozoa

	Subunit composition and function of GABA(A) receptors of rat spermatozoa
	Hu, JH; He, XB; Wu, Q; Yan, YC; Koide, SS
刊名	NEUROCHEMICAL RESEARCH
	2002
卷号	27 期号:3 页码:195-199
关键词	acrosome reaction GABA(A) receptor agonist and antagonist chlortetracycline assay progesterone
通讯作者	Yan, YC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	GABA triggers mammalian sperm acrosome reaction (AR). Here, evidence is presented, showing that rat spermatozoa contain GABA(A) receptors, composed of alpha(5), beta(1), and beta(3) subunits. The effects of GABA(A) receptor agonist and antagonist on the induction of AR in rat spermatozoa were assessed using the chlortetracycline assay. Muscimol, a GABA(A) receptor agonist, triggered AR; whereas bicuculline, a GABA(A) receptor antagonist and picrotoxin, a GABA(A) receptor/Cl- channel blocker, inhibited the ability of GAGA or progesterone to induce AR. In conclusion, GABA(A) receptors appear to mediate the action of progesterone in inducing AR in rat spermatozoa.
学科主题	Biochemistry & Molecular Biology; Neurosciences & Neurology
类目[WOS]	Biochemistry & Molecular Biology ; Neurosciences
关键词[WOS]	GAMMA-AMINOBUTYRIC-ACID ; SPERM ACROSOME REACTION ; MOUSE SPERMATOZOA ; BINDING-SITES ; PROGESTERONE ; EXPRESSION ; CHLORTETRACYCLINE ; CAPACITATION ; EXOCYTOSIS ; BRAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000174854100003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2472]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, JH,He, XB,Wu, Q,et al. Subunit composition and function of GABA(A) receptors of rat spermatozoa[J]. NEUROCHEMICAL RESEARCH,2002,27(3):195-199.
APA	Hu, JH,He, XB,Wu, Q,Yan, YC,&Koide, SS.(2002).Subunit composition and function of GABA(A) receptors of rat spermatozoa.NEUROCHEMICAL RESEARCH,27(3),195-199.
MLA	Hu, JH,et al."Subunit composition and function of GABA(A) receptors of rat spermatozoa".NEUROCHEMICAL RESEARCH 27.3(2002):195-199.