Growth inhibition of BEL-7404 human hepatoma cells by expression of mutant telomerase reverse transcriptase

	Growth inhibition of BEL-7404 human hepatoma cells by expression of mutant telomerase reverse transcriptase
	Zhang, RG; Wang, XW; Guo, LX; Xie, H
刊名	INTERNATIONAL JOURNAL OF CANCER
	2002
卷号	97 期号:2 页码:173-179
关键词	hepatocellular carcinoma telomerase DN-hTERT cell growth tumorigenicity
通讯作者	Xie, H (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Dept Biotherapy, Cell Bldg, Shanghai 200031, Peoples R China.,
英文摘要	Human hepatocellular carcinoma (HCC) is one of the most common malignancies in Asia and Africa. Human telomerase reverse transcriptase (hTERT) is expressed in HCC but absent in normal human liver cells, which is consistent with the expression pattern of telomerase. In the present study, expression of a dominant-negative form of hTERT (DN-hTERT) resulted in inhibition of telomerase activity and decreased mean telomeric length of BEL-7404 human hepatoma cells, whereas expression of wild-type hTERT (WT-hTERT) and control vector had no such effects. Cell growth was inhibited by this mutant (DN-hTERT), which was consistent with the changes in telomerase level. Flattened large cells were found in late generations with the DN-hTERT treatment. When mean telomeric length of DN-hTERT-transfected cells reached a critical length (about 1.7 kb), apoptosis was induced. Tumorigenicity of DN-hTERT-expressing cells was eliminated in vivo. These data indicated that hTERT was essential for the growth of hepatoma cells. hTERT can also be used as an important target for anti-HCC drug screening. (C) 2002 Wiley-Liss, Inc.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	HUMAN LIVER-TISSUES ; HUMAN TUMOR-CELLS ; HEPATOCELLULAR-CARCINOMA ; IN-VITRO ; HAMMERHEAD RIBOZYME ; INTERACTIVE AGENTS ; CATALYTIC SUBUNIT ; RNA COMPONENT ; CANCER ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000172877900007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2452]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, RG,Wang, XW,Guo, LX,et al. Growth inhibition of BEL-7404 human hepatoma cells by expression of mutant telomerase reverse transcriptase[J]. INTERNATIONAL JOURNAL OF CANCER,2002,97(2):173-179.
APA	Zhang, RG,Wang, XW,Guo, LX,&Xie, H.(2002).Growth inhibition of BEL-7404 human hepatoma cells by expression of mutant telomerase reverse transcriptase.INTERNATIONAL JOURNAL OF CANCER,97(2),173-179.
MLA	Zhang, RG,et al."Growth inhibition of BEL-7404 human hepatoma cells by expression of mutant telomerase reverse transcriptase".INTERNATIONAL JOURNAL OF CANCER 97.2(2002):173-179.