Mouse restin inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis

	Mouse restin inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis
	Ren, X; Li, X; Yi, F; Meng, HR; Li, ZP; Gan, RB
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2002
卷号	34 期号:2 页码:138-142
关键词	restin endostatin collagen XV angiogenesis inhibitor apoptosis
通讯作者	,
英文摘要	Restin, a homologous protein of endostatin, was found by Ramchandran et al. It was the C-terminal fragment of type XV collagen. To analysis the inhibition activity of mouse restin on the proliferation of endothelial cells, the cDNA of restin was amplified from the total RNA of the mouse muscle and cloned into the prokaryotic expression plasmid pQE32. The recombinant protein was expressed in inclusion body with a yield about 60%-70% of total protein. After refolding, the purified recombinant protein specifically inhibits bovine aortic endothelial (BAE) cell proliferation stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner, but the activity of restin was weaker than that of endostatin. Treatment of BAE cell with recombinant restin caused G, arrest and apoptosis in BAE cells.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	HUMAN ENDOSTATIN ; TUMOR-GROWTH ; COLLAGEN ; EXPRESSION ; CLONING ; XV ; ANGIOGENESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000174549400004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2447]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ren, X,Li, X,Yi, F,et al. Mouse restin inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(2):138-142.
APA	Ren, X,Li, X,Yi, F,Meng, HR,Li, ZP,&Gan, RB.(2002).Mouse restin inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(2),138-142.
MLA	Ren, X,et al."Mouse restin inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.2(2002):138-142.