Identification of alternatively spliced Act1 and implications for its roles in oncogenesis

	Identification of alternatively spliced Act1 and implications for its roles in oncogenesis
	Xia, YF; Li, YD; Li, XX; Geng, JG
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2002
卷号	296 期号:2 页码:406-412
关键词	Act1 alternative splicing NF-kappa B AP-1 oncogenesis inflammation
通讯作者	Geng, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Act1 (also called CIKS) is a recently identified molecule, which activates NF-kappaB and AP-1 Here, we identified alternatively spliced Act1 that lacked the exon 2 encoding the first nine amino acids in the amino terminus of the protein. Compared to full-length Act1, this truncated Act1 appeared to be equally active. We demonstrated further that only the spliced Act1 was detected in cDNA libraries derived from human fetal brain, liver, leukocytes, and bone marrow. In contrast, both the spliced and full-length Act1 templates were detected in a variety of human cancer cell lines. The expression of both the spliced and Full-length transcripts was detected at 4-h time point, following the treatment of endothelial cells with tumor necrosis factor-alpha, interleukin-1beta or bacterial endotoxin. Notably. the dominant amounts of the spliced Act1 over the full-length Act1 were amplified from both the cancer cell mRNAs and the stimulated endothelial cell mRNAs. Taken together with the act1 chromosome localization Lit the 6q21 subregion, our findings indicate that the newly identified alternatively spliced Act1 is a major transcript of the molecule and that Act1 may play important roles in oncogenesis. (C) 2002 Elsevier Science (USA). All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	NF-KAPPA-B ; E-SELECTIN ; CELLS ; ADHESION ; PROTEINS ; ANGIOGENESIS ; EXPRESSION ; ACTIVATION ; PATHWAY ; CANCER
收录类别	SCI
语种	英语
WOS记录号	WOS:000177525800028
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2434]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xia, YF,Li, YD,Li, XX,et al. Identification of alternatively spliced Act1 and implications for its roles in oncogenesis[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,296(2):406-412.
APA	Xia, YF,Li, YD,Li, XX,&Geng, JG.(2002).Identification of alternatively spliced Act1 and implications for its roles in oncogenesis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,296(2),406-412.
MLA	Xia, YF,et al."Identification of alternatively spliced Act1 and implications for its roles in oncogenesis".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 296.2(2002):406-412.