Human interleukin 10 gene therapy decreases the severity and mortality of lethal pancreatitis in rats

	Human interleukin 10 gene therapy decreases the severity and mortality of lethal pancreatitis in rats
	Zou, WG; Wang, DS; Lang, MF; Jin, DY; Xu, DH; Zheng, ZC; Wu, ZH; Liu, XY
刊名	JOURNAL OF SURGICAL RESEARCH
	2002
卷号	103 期号:1 页码:121-126
关键词	acute pancreatitis liposomes hIL-10 gene therapy
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,
英文摘要	Background. Studies have proven the validity of interleukin-10 (IL-10) in the treatment of experimental pancreatitis. Prophylactic human IL-10 (hIL-10) gene treatment attenuated the severity in cerulein models. Our research aims to study whether the therapeutic hIL-10 gene could decrease both severity and mortality in a lethal pancreatic model. Methods. Severe acute pancreatitis (SAP) was induced by sodium taurocholate. A plasmid-hIL-10 construct (pcDNA3-hIL-10) complexed with cationic liposomes was administered to SAP rats by a single intraperitoneal injection. Levels of hIL-10 in the pancreas, liver, and lungs were determined by ELISA kits. The severity of pancreatitis was assessed in terms of serum amylase, histology, and tissue tumor necrosis factor a (TNF-alpha). Mortality, observed for 7 days, was evaluated for gene therapy or control groups. Results. After hIL-10 gene therapy, hIL-10 levels in the pancreas, liver, and lungs increased significantly and the serum amylase, tissue TNF-alpha, and histological changes in pancreas, liver, and lungs decreased markedly. Therefore, mortality was significantly reduced in the hIL-10 gene therapy group, in which 70% of rats survived in the 7-day observation, while only 10% survived in untreated groups (P < 0.05). Conclusion. We found that liposome/hIL-10 gene therapy decreased severity and mortality in SAP, even carried out after SAP establishment, predicting a more convenient shift to clinical applications.
学科主题	Surgery
类目[WOS]	Surgery
关键词[WOS]	INHIBITS CYTOKINE PRODUCTION ; NECROSIS-FACTOR-ALPHA ; INFLAMMATORY MEDIATORS ; CELLS ; DEATH ; ACTIVATION ; RELEASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000174189400018
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2421]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zou, WG,Wang, DS,Lang, MF,et al. Human interleukin 10 gene therapy decreases the severity and mortality of lethal pancreatitis in rats[J]. JOURNAL OF SURGICAL RESEARCH,2002,103(1):121-126.
APA	Zou, WG.,Wang, DS.,Lang, MF.,Jin, DY.,Xu, DH.,...&Liu, XY.(2002).Human interleukin 10 gene therapy decreases the severity and mortality of lethal pancreatitis in rats.JOURNAL OF SURGICAL RESEARCH,103(1),121-126.
MLA	Zou, WG,et al."Human interleukin 10 gene therapy decreases the severity and mortality of lethal pancreatitis in rats".JOURNAL OF SURGICAL RESEARCH 103.1(2002):121-126.