Identification of gamma(1) subunit of GABA(A) receptor in rat testis

	Identification of gamma(1) subunit of GABA(A) receptor in rat testis
	Hu, JH; Yan, YC
刊名	CELL RESEARCH
	2002
卷号	12 期号:1 页码:33-37
关键词	GABA(A) receptor rat testis gamma(1) subunit
通讯作者	Yan, YC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,
英文摘要	The isoform type of gamma subunits of GABA(A) receptor is a molecular determinant of its pharmacological characteristics. At present, the existence of GABA(A) receptor in mammalian sperm is still a controversy. By using degenerate primers designed according to highly conserved region in all three gamma (gamma(1), gamma(2) and gamma(3)) subunits cloned in rat brain, we performed reverse transcription polymerase chain reaction (RT-PCR) to examine the expression pattern of gamma subunits of GABA(A) receptor in rat testis. Only one 370 bp fragment was obtained from RT-PCR in rat testis and sequencing results showed that it represented gamma(1) subunit, but not gamma(2) or gamma(3) subunit. Using the cloned fragment as probe, a 3.8 kb transcript which in size as same as gamma(1) subunit in rat brain was detected in rat testis mRNA by performing Northern blot assay. Furthermore, results of in situ hybridization assay confirmed that gamma(1) subunit was expressed in round spermatids and spermatozoa, maybe also in secondary spermatocyte. These evidences proved that gamma(1) subunit of GABA(A) receptor is exclusively expressed in rat testis and this feature may be the structural basis of the specific function of GABA(A) receptors in sperm acrosome reaction.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	SPERM PLASMA-MEMBRANE ; AMINOBUTYRIC-ACID ; ACROSOME REACTION ; A-RECEPTOR ; MESSENGER-RNAS ; PROGESTERONE ; BRAIN ; SPERMATOZOA ; EXPRESSION ; CHANNELS
收录类别	SCI
语种	英语
WOS记录号	WOS:000174833300004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2416]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hu, JH,Yan, YC. Identification of gamma(1) subunit of GABA(A) receptor in rat testis[J]. CELL RESEARCH,2002,12(1):33-37.
APA	Hu, JH,&Yan, YC.(2002).Identification of gamma(1) subunit of GABA(A) receptor in rat testis.CELL RESEARCH,12(1),33-37.
MLA	Hu, JH,et al."Identification of gamma(1) subunit of GABA(A) receptor in rat testis".CELL RESEARCH 12.1(2002):33-37.