Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells

	Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells
	Tang, K; Yang, J; Gao, X; Wang, C; Liu, L; Kitani, H; Atsumi, T; Jing, N
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2002
卷号	293 期号:1 页码:167-173
关键词	Wnt-1 P19 embryonic carcinoma cell neural differentiatiom neuronal glial bHLH genes Mash-1 Ngn-1
通讯作者	Jing, N (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Wnt-1, the vertebrate counterpart of the Drosophila wingless gene, plays an important role in the early morphogenesis of neural tissues. In this report, we have shown that overexpression of Wnt-1 can direct embryonic carcinoma P19 cells to differentiate into neuron-like cells in the absence of retinoic acid. Immunocytochemistry showed that these cells expressed neuronal markers, such as the neurofilament (NF) and micro tubule-associated protein 2 (MAP2), but failed to express the glial cell marker, glial fibrillary acidic protein (GFAP). RT-PCR revealed that two basic helix-loop-helix (bHLH) genes. Mash-1 and Ngn-1, were up-regulated during the differentiation stage of Wnt-1-overexpressing P19 cells. These results suggest that the Wnt-1 gene promotes neuronal differentiation and inhibits gliogenesis during the neural differentiation of P19 cells, and that neural bHLH genes might be involved in this process. (C) 2002 Elsevier Science (USA). All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	EMBRYONAL CARCINOMA-CELLS ; MAMMALIAN ACHAETE-SCUTE ; CENTRAL-NERVOUS-SYSTEM ; NEUROECTODERMAL DIFFERENTIATION ; TRANSIENT EXPRESSION ; INT-1 PROTOONCOGENE ; NEURAL DEVELOPMENT ; ONCOGENE INT-1 ; PROTO-ONCOGENE ; STEM-CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000175514700027
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2414]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Tang, K,Yang, J,Gao, X,et al. Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,293(1):167-173.
APA	Tang, K.,Yang, J.,Gao, X.,Wang, C.,Liu, L.,...&Jing, N.(2002).Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,293(1),167-173.
MLA	Tang, K,et al."Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 293.1(2002):167-173.