The binding of BmK abT, a unique neurotoxin, to mammal brain and insect Na+ channels using biosensor

	The binding of BmK abT, a unique neurotoxin, to mammal brain and insect Na+ channels using biosensor
	Ji, YH; Wang, WX; Wang, Q; Huang, YP
刊名	EUROPEAN JOURNAL OF PHARMACOLOGY
	2002
卷号	454 期号:1 页码:25-30
关键词	scorpion neurotoxin BmK abT Na+ channel receptor binding site BIAcore assay
通讯作者	Ji, YH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,yhji@server.shcnc.ac.cn
英文摘要	The binding properties of BmK abT (a novel neurotoxic polypeptide abT from Chinese scorpion Buthus martensi Karsch), a unique neurotoxin from Chinese scorpion, on mammal brain and insect sodium channels were investigated using the BIAcore assay. Results showed that BmK abT could bind to rat brain synaptosomes with an association rate constant of about 2.49 x 10(6) M-1 s(-1) and a dissociation rate constant of about 1.57 x 10(-4) s(-1), and to Heliothis nerve cord synaptosomes with an association rate constant of about 1.21 x 10(7) M-1 s(-1) and a dissociation rate constant of about 0.99 x 10(-3) s(-1). The binding of BmK abT to rat brain synaptosomes could be partially inhibited by increasing the membrane potential, but not by BmK AS (a novel active polypeptide AS from B. martensi Karsch), BmK IT2 (a depressant insect-selective toxin IT2 from B. martensi Karsch), and BmK I (an alpha-like anti-mammal toxin I from B. martensi Karsch). Binding was not modulated by veratridine. In addition, the binding of BmK abT to Heliothis nerve cord synaptosomes was significantly enhanced by increasing the membrane potential and veratridine concentration and was inhibited by BmK IT2, but not by BmK AS or BmK I. The results suggest that BmK abT binds to a distinct receptor site on mammal brain Na+ channels and associates with a related site for depressant insect-selective toxins on insect sodium channels. (C) 2002 Elsevier Science B.V All rights reserved.
学科主题	Pharmacology & Pharmacy
类目[WOS]	Pharmacology & Pharmacy
关键词[WOS]	BUTHUS-MARTENSI KARSCH ; GATED SODIUM-CHANNELS ; SELECTIVE SCORPION TOXIN ; RAT-BRAIN ; RECEPTOR-SITES ; VENOM ; SYNAPTOSOMES ; IT2 ; PURIFICATION ; POLYPEPTIDE
收录类别	SCI
语种	英语
WOS记录号	WOS:000179057900004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2406]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ji, YH,Wang, WX,Wang, Q,et al. The binding of BmK abT, a unique neurotoxin, to mammal brain and insect Na+ channels using biosensor[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2002,454(1):25-30.
APA	Ji, YH,Wang, WX,Wang, Q,&Huang, YP.(2002).The binding of BmK abT, a unique neurotoxin, to mammal brain and insect Na+ channels using biosensor.EUROPEAN JOURNAL OF PHARMACOLOGY,454(1),25-30.
MLA	Ji, YH,et al."The binding of BmK abT, a unique neurotoxin, to mammal brain and insect Na+ channels using biosensor".EUROPEAN JOURNAL OF PHARMACOLOGY 454.1(2002):25-30.