A specific splicing variant of SVH, a novel human armadillo repeat protein, is up-regulated in hepatocellular carcinomas

	A specific splicing variant of SVH, a novel human armadillo repeat protein, is up-regulated in hepatocellular carcinomas
	Huang, RM; Xing, ZG; Luan, ZD; Wu, TM; Wu, X; Hu, GX
刊名	CANCER RESEARCH
	2003
卷号	63 期号:13 页码:3775-3782
通讯作者	Hu, GX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Cell Biol Bldg,Room 400,320 Yueyang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with poor prognosis. By representational difference analysis (RDA), a novel human gene designated SVH, up-regulated in the clinical HCC sample, was identified. The deduced SVH protein consisted of 343 amino acids with a transmembrane domain and an armadillo repeat. Northern blot revealed that SVH was expressed in most human adult tissues. Four variants of SVH, SVH-A, -B, -C, and -D, resulting from alternative splicing in the coding region of the SVH transcript, were observed and were all localized in endoplasmic reticulum (ER). Upregulation of SVH-B, but not the other variants, was evident in about 60% (28 of 46) of HCC samples, detected by quantitative real-time PCR. Human liver cell line QSG-7701, transfected with SVH-B, acquired an accelerated growth rate and tumorigenicity in nude mice, whereas inhibition of SVH-B in hepatoma cell line BEL-7404, using antisense oligode-oxynucleotides, induced apoptosis. It is suggested that the splicing variants of SVH have distinct biological functions, and SVH-B may play an important role in hepatocarcinogenesis.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	PRE-MESSENGER-RNA ; ENDOPLASMIC-RETICULUM ; DELTA-CATENIN ; CELL-DEATH ; CANCER ; TUMOR ; GENES ; CD44 ; LYMPHOCYTES ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000183941800051
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2371]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Huang, RM,Xing, ZG,Luan, ZD,et al. A specific splicing variant of SVH, a novel human armadillo repeat protein, is up-regulated in hepatocellular carcinomas[J]. CANCER RESEARCH,2003,63(13):3775-3782.
APA	Huang, RM,Xing, ZG,Luan, ZD,Wu, TM,Wu, X,&Hu, GX.(2003).A specific splicing variant of SVH, a novel human armadillo repeat protein, is up-regulated in hepatocellular carcinomas.CANCER RESEARCH,63(13),3775-3782.
MLA	Huang, RM,et al."A specific splicing variant of SVH, a novel human armadillo repeat protein, is up-regulated in hepatocellular carcinomas".CANCER RESEARCH 63.13(2003):3775-3782.