Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii

	Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii
	Li, F; Yang, XX; Hu, WG; Xia, HC; Li, Z; Zhang, ZC
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2003
卷号	35 期号:9 页码:841-846
关键词	trichokirin-S1 translational inhibitory activity rRNA N-glycosidase
通讯作者	Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai 200031, Peoples R China.,
英文摘要	A novel peptide from the seeds of Trichosanthes kirilowii, trichokirin-S1, was purified by extraction of protein body, ammonia sulfate precipitation, Blue-gel affinity chromatography, FPLC Mono S ion exchange chromatography and Superose12 gel filtration chromatography. Its molecular weight was determined to be 11 426 by MALDI-TOF MS analysis. Its reaction mechanism to inactive ribosome was the same as that of the ribosome-inactivating protein trichosanthin, a rRNA N-glycosidase. The purified trichokirin-SI showed a strong inhibitory activity on protein synthesis in cell-free rabbit reticulocyte lysate system, with IC50 of 0.7 nmol/L. Therefore, trichokirin-SI may be a promising and efficient toxin moiety of immunotoxins.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	TRANSLATION-INHIBITING ACTIVITY ; VACUOLAR PROCESSING ENZYME ; BETA-KIRILOWIN ; PROTEIN ; MECHANISM
收录类别	SCI
语种	中文
WOS记录号	WOS:000185493600011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2343]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, F,Yang, XX,Hu, WG,et al. Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(9):841-846.
APA	Li, F,Yang, XX,Hu, WG,Xia, HC,Li, Z,&Zhang, ZC.(2003).Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(9),841-846.
MLA	Li, F,et al."Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.9(2003):841-846.