Study on the solution conformation of orphanin FQ

	Study on the solution conformation of orphanin FQ
	He, FH; Zhang, Q; Shi, JH; Cui, DF; Wu, HM
刊名	ACTA CHIMICA SINICA
	2003
卷号	61 期号:7 页码:1101-1107
关键词	orphanin FQ 2D-NMR secondary structure solution conformation
通讯作者	Wu, HM (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, 345 Lingling Lu, Shanghai 200032, Peoples R China.,
英文摘要	A comparative study on the solution conformation between OFQ and its fragment OFQ(8similar to17) Was Carried Out using 2D NMR experiments both in H2O and TFE/H2O. After completion of amino acid spin system recognition and the sequence-specific assignments, the regular secondary structure of OFQ and OFQ(8similar to17) in different solvents were deduced from NOE patterns, and backbone coupling constants ((3)J(HN-Halpha)). The solution structure of OFQ both in H2O and TFE/H2O was obtained using distance constraints derived from NOE cross peaks and dihedral angles constraints and was refined by DG, RMD and SA calculations. The structural feature of OFQ in water was characterized by a mixing type turn in segment of Gly3-Phe4-The5-Gly6 at 278 K. While the conformation of OFQ was identified as an amphipathic alpha-helix in TFE/H2O. However, OFQ(8similar to17) demonstrated random conformation both in water and in TFE/H2O. These results revealed that OFQ assumes amphipathic helical conformation in the membrane-like circumstances involved in the interaction with the receptor.
学科主题	Chemistry
类目[WOS]	Chemistry, Multidisciplinary
关键词[WOS]	OPIOID RECEPTOR FAMILY ; CLONING ; MEMBER ; RAT
收录类别	SCI
语种	中文
WOS记录号	WOS:000184988400025
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2325]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	He, FH,Zhang, Q,Shi, JH,et al. Study on the solution conformation of orphanin FQ[J]. ACTA CHIMICA SINICA,2003,61(7):1101-1107.
APA	He, FH,Zhang, Q,Shi, JH,Cui, DF,&Wu, HM.(2003).Study on the solution conformation of orphanin FQ.ACTA CHIMICA SINICA,61(7),1101-1107.
MLA	He, FH,et al."Study on the solution conformation of orphanin FQ".ACTA CHIMICA SINICA 61.7(2003):1101-1107.