cDNA cloning of two A-superfamily conotoxins from Conus striatus

	cDNA cloning of two A-superfamily conotoxins from Conus striatus
	Wang, CZ; Jiang, H; Ou, ZL; Chen, JS; Chi, CW
刊名	TOXICON
	2003
卷号	42 期号:6 页码:613-619
关键词	conotoxin Conus striatus Conus peptide cDNA cloning
通讯作者	Chi, CW (reprint author), Chinese Acad Sci, Key Lab Proteom, Shanghai Inst Biol Sci, Shanghai Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	The full-length cDNAs of two A-superfamily conotoxins, kappaA-SIVA and alpha-SII, were respectively cloned and sequenced from Conus striatus using 3' RACE and 5' RACE. The cDNA of kappaA-SIVA encodes a precursor of 68 residues, including a signal peptide of 21 residues, a pro-peptide of 17 residues, and a mature peptide of 30 residues with an additional residue Gly which is prerequisite for the amidation of the preceding C-terminal Cys. The cDNA-deduced sequence of alpha-SII is composed of a signal peptide of 21 residues, a pro-peptide of 29 residues, a mature peptide of 19 residues and three additional residues Arg-Thr-Ile at the C-terminus. This tripeptide might be cleaved off by proteolytic processing. Although these two conotoxins belong to different families and target voltage-gated potassium channel and nicotinic acetylcholine receptor, respectively, they share the same signal sequence, and both are processed at the common signal site -X-Arg- immediately before the mature peptide sequences. The length of 3' untranslational region of a-conotoxin SII was extraordinarily large about 10 times longer than that of kappaA-SIVA with 770 and 75 bp, respectively. The elucidated cDNAs of these two toxins will facilitate a better understanding of the process of their post-translational modifications. (C) 2003 Elsevier Ltd. All rights reserved.
学科主题	Pharmacology & Pharmacy; Toxicology
类目[WOS]	Pharmacology & Pharmacy ; Toxicology
关键词[WOS]	BUTHUS-MARTENSI KARSCH ; NICOTINIC ACETYLCHOLINE-RECEPTORS ; GENOMIC ORGANIZATION ; POTASSIUM CHANNELS ; OMEGA-CONOTOXIN ; ALPHA-CONOTOXIN ; SODIUM-CHANNELS ; ION-CHANNEL ; PEPTIDE ; SNAILS
收录类别	SCI
语种	英语
WOS记录号	WOS:000186673300005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2313]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, CZ,Jiang, H,Ou, ZL,et al. cDNA cloning of two A-superfamily conotoxins from Conus striatus[J]. TOXICON,2003,42(6):613-619.
APA	Wang, CZ,Jiang, H,Ou, ZL,Chen, JS,&Chi, CW.(2003).cDNA cloning of two A-superfamily conotoxins from Conus striatus.TOXICON,42(6),613-619.
MLA	Wang, CZ,et al."cDNA cloning of two A-superfamily conotoxins from Conus striatus".TOXICON 42.6(2003):613-619.