The heparan sulfate proteoglycan GPC3 is a potential lung tumor suppressor

	The heparan sulfate proteoglycan GPC3 is a potential lung tumor suppressor
	Kim, H; Xu, GL; Borczuk, AC; Busch, S; Filmus, J; Capurro, M; Brody, JS; Lange, J; D'Armiento, JM; Rothman, PB
刊名	AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
	2003
卷号	29 期号:6 页码:694-701
通讯作者	Powell, CA (reprint author), Columbia Univ, Div Pulm Allergy & Crit Care Med, 630 W 168th St,Box 91, New York, NY 10032 USA.,
英文摘要	Recently, we used gene expression profiling of lung adenocarcinoma and paired normal tissue from smokers and nonsmokers to identify genes and molecular pathways associated with cigarette smoking and lung carcinogenesis. The gene encoding Glypican 3, a glycosylphosphatidylinositol-linked heparan sulfate proteoglycan, was decreased in lung adenocarcinoma. Within nonmalignant lung, GPC3 expression was decreased in smokers compared with nonsmokers; indicating that expression is associated with cigarette smoking. Microarray results were confirmed using an independent cohort of tumors and nonmalignant lung tissues. Immunohistochemical studies localized Glypican 3 protein expression to the apical surface of lung bronchiolar epithelial cells, potential cells of origin for adenocarcinoma. Northern blot analysis demonstrated expression was absent in all tested non-small cell lung carcinoma lines. Pharmacologic treatment of lung cell lines indicated that GPC3 expression was epigenetically silenced by promoter hypermethylation. Human lung carcinoma tumor cells ectopically expressing GPC3 demonstrated increased apoptosis response when exposed to etoposide and growth inhibition when implanted in nude mice. These findings suggest that GPC3 is a candidate lung tumor suppressor gene whose expression may be regulated by exposure to cigarette smoke and functions to modulate cellular response to exogenous damage.
学科主题	Biochemistry & Molecular Biology; Cell Biology; Respiratory System
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology ; Respiratory System
关键词[WOS]	GOLABI-BEHMEL-SYNDROME ; ABERRANT PROMOTER METHYLATION ; GENE-EXPRESSION ; BREAST-CANCER ; BRONCHIAL EPITHELIUM ; OVERGROWTH ; CELLS ; ADENOCARCINOMA ; ABNORMALITIES ; INHIBITION
收录类别	SCI
语种	英语
WOS记录号	WOS:000187175400006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2309]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Kim, H,Xu, GL,Borczuk, AC,et al. The heparan sulfate proteoglycan GPC3 is a potential lung tumor suppressor[J]. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY,2003,29(6):694-701.
APA	Kim, H.,Xu, GL.,Borczuk, AC.,Busch, S.,Filmus, J.,...&Powell, CA.(2003).The heparan sulfate proteoglycan GPC3 is a potential lung tumor suppressor.AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY,29(6),694-701.
MLA	Kim, H,et al."The heparan sulfate proteoglycan GPC3 is a potential lung tumor suppressor".AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY 29.6(2003):694-701.