Expression of hepatitis C virus hypervariable region 1 and its clinical significance

	Expression of hepatitis C virus hypervariable region 1 and its clinical significance
	Zhang, XX; Zhang, SY; Liu, J; Lu, ZM; Wang, Y
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2003
卷号	9 期号:5 页码:1003-1007
通讯作者	Wang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	AIM: To explore the properties of hypervariable region 1 (HVR1) in the envelope 2 gene of hepatitis C virus by analyzing the reactivity of HVR1 fusion proteins from different Chinese HCV strains with sera of patients with chronic hepatitis C and by comparing their reactivity between interferon therapy responders and non-responders. METHODS: Gene fragments of HVR1 of four HCV strains (three genotype 1b and one genotype 2a) were amplified from pGEMT-E2 plasmids and sub-cloned into pQE40 vectors respectively to construct recombinant expression plasmids which expressed HVR1 fused downstream to DHFR in Escherichia coli strain TG1. The purified DHFR-HVR1 proteins were then used to detect the anti-HVR1 antibodies in 70 serum samples of patients with chronic hepatitis C. RESULTS: Four DHFR-HVR1 fusion proteins were successfully expressed in E.coli (320-800 ug fusion proteins per 100 ml culture). Each fusion protein (SH1b, BJ1b, SD1b and SD2a) reacted with 72.8 % (51/70), 60 % (42/70), 48.6 % (34/70), and 58.6 % (41/70) of the anti-HCV positive patients' sera respectively by ELISA. 57.1 % (4/7) of non responders reacted with all four HVR1 fusion proteins, while only 15.3 % (2/13) of responders reacted with all of them. The O.D. values of sera from IFN therapy responders were significantly higher than those of non responders (P<0.05). CONCLUSION: The selected HVR1 fusion proteins expressed in E. coli can broadly react with HCV-infected patients' sera. The intensity and/or quality of the immune response against HCV may be a critical factor determining the response to interferon treatment. With the evolution of virus strains, anti-HVR1 antibodies can not neutralize all the quasispecies. A polyvalent and high immunogenic vaccine comprising a mixture of several HVR1 sequences that cover the reactivity of most HCV isolates may be useful.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
关键词[WOS]	INTERFERON THERAPY ; GENETIC-HETEROGENEITY ; REACTIVE ANTIBODIES ; CROSS-REACTIVITY ; E2 PROTEIN ; HCV GENOME ; INFECTION ; ALPHA ; VACCINATION ; CHIMPANZEES
收录类别	SCI
语种	英语
WOS记录号	WOS:000183039900023
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2308]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, XX,Zhang, SY,Liu, J,et al. Expression of hepatitis C virus hypervariable region 1 and its clinical significance[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(5):1003-1007.
APA	Zhang, XX,Zhang, SY,Liu, J,Lu, ZM,&Wang, Y.(2003).Expression of hepatitis C virus hypervariable region 1 and its clinical significance.WORLD JOURNAL OF GASTROENTEROLOGY,9(5),1003-1007.
MLA	Zhang, XX,et al."Expression of hepatitis C virus hypervariable region 1 and its clinical significance".WORLD JOURNAL OF GASTROENTEROLOGY 9.5(2003):1003-1007.