Analysis of germ cell nuclear factor transcripts and protein expression during spermatogenesis

	Analysis of germ cell nuclear factor transcripts and protein expression during spermatogenesis
	Yang, GY; Zhang, YL; Buchold, GM; Jetten, AM; O'Brien, DA
刊名	BIOLOGY OF REPRODUCTION
	2003
卷号	68 期号:5 页码:1620-1630
关键词	GCNF gene regulation nuclear orphan receptor spermatogenesis testis
通讯作者	O'Brien, DA (reprint author), Univ N Carolina, Dept Cell & Dev Biol, Sch Med, Labs Reprod Biol, CB 7090,214A Taylor Hall, Chapel Hill, NC 27599 USA.,
英文摘要	Germ cell nuclear factor (GCNF), an orphan receptor in the nuclear receptor superfamily, is expressed predominantly in developing germ cells in the adult mouse. Two Gcnf transcripts (7.4 and 2.1 kilobase [kb]) encoded by a single copy gene are expressed in the testis of several mammalian species. To identify features that regulate Gcnf expression, we characterized the structure and sequence of the mouse gene and its two transcripts and determined the expression profile of the GCNF protein during spermatogenesis. Genomic fragments spanning part of the 5'-untranslated region (UTR), the coding sequence, and the complete 3'-UTR (similar to80 kb) were isolated and sequenced. The 3'-UTRs of the two transcripts are quite distinct. The 7.4 kb transcript, which appears earlier in spermatogenesis, has a very long 3'-UTR of 4451 nucleotides. In contrast, the 2.1 kb transcript, which is expressed predominantly during the haploid phase of spermatogenesis, has a 3'-UTR that is only 202 nucleotides in length. Additional analyses indicate that both transcripts share the same coding region and are associated with polysomes. A single GCNF protein band was detected in testis extracts by Western blotting with a specific antiserum. Immunohistochemical analysis showed that GCNF is localized in the nuclei of pachytene spermatocytes and round spermatids. GCNF is first detectable in early pachytene spermatocytes (stage II) and is continuously expressed until spermatids begin to elongate in stage IX. Although GCNF is generally distributed throughout the nucleus, it is particularly prominent in heterochromatic regions at some stages and in condensed chromosomes undergoing the meiotic divisions. This expression profile suggests that GCNF plays a role in transcriptional regulation during meiosis and the early haploid phase of spermatogenesis.
学科主题	Reproductive Biology
类目[WOS]	Reproductive Biology
关键词[WOS]	TESTIS-ASSOCIATED RECEPTOR ; ORPHAN RECEPTOR ; MESSENGER-RNA ; DNA-BINDING ; TRANSLATIONAL REGULATION ; DIFFERENTIAL EXPRESSION ; MOUSE SPERMIOGENESIS ; RESPONSE ELEMENT ; MAMMALIAN TESTIS ; XENOPUS-LAEVIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000182589700019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2304]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, GY,Zhang, YL,Buchold, GM,et al. Analysis of germ cell nuclear factor transcripts and protein expression during spermatogenesis[J]. BIOLOGY OF REPRODUCTION,2003,68(5):1620-1630.
APA	Yang, GY,Zhang, YL,Buchold, GM,Jetten, AM,&O'Brien, DA.(2003).Analysis of germ cell nuclear factor transcripts and protein expression during spermatogenesis.BIOLOGY OF REPRODUCTION,68(5),1620-1630.
MLA	Yang, GY,et al."Analysis of germ cell nuclear factor transcripts and protein expression during spermatogenesis".BIOLOGY OF REPRODUCTION 68.5(2003):1620-1630.