Protein engineering of insulin: Two novel fast-acting insulins [B16Ala]insulin and [B26Ala]insulin

	Protein engineering of insulin: Two novel fast-acting insulins [B16Ala]insulin and [B26Ala]insulin
	Zhang, Z; Tang, YH; Yao, SY; Zhu, SQ; Feng, YM
刊名	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
	2003
卷号	46 期号:5 页码:474-480
关键词	insulin monomeric insulin fast-acting insulin Pichia pastoris
通讯作者	Feng, YM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,
英文摘要	Blood glucose lowering assay proved that [B16Ala]insulin and [B26Ala]insulin exhibit potency of acute blood glucose lowering in normal pigs, which demonstrates that they are fast-acting insulin. Single-chain precursor of [B16Ala]insulin and [B26Ala]insulin is [B16Ala]PIP and [B26Ala]PIP, respectively, which are suitable for gene expression. Secretory expression level of the precursors in methylotrophic yeast Pichia pastoris was quite high, 650 mg/L and 130 mg/L, respectively. In vivo biological assay showed that the two fast-acting insulins have full or nearly full biological activity. So both [B16Ala]insulin and [B26Ala]insulin can be well developed as fast-acting insulin for clinic use.
学科主题	Life Sciences & Biomedicine - Other Topics
类目[WOS]	Biology
关键词[WOS]	ABSORPTION KINETICS ; RESOLUTION ; ANALOG
收录类别	SCI
语种	英语
WOS记录号	WOS:000185764100003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2298]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, Z,Tang, YH,Yao, SY,et al. Protein engineering of insulin: Two novel fast-acting insulins [B16Ala]insulin and [B26Ala]insulin[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2003,46(5):474-480.
APA	Zhang, Z,Tang, YH,Yao, SY,Zhu, SQ,&Feng, YM.(2003).Protein engineering of insulin: Two novel fast-acting insulins [B16Ala]insulin and [B26Ala]insulin.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,46(5),474-480.
MLA	Zhang, Z,et al."Protein engineering of insulin: Two novel fast-acting insulins [B16Ala]insulin and [B26Ala]insulin".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 46.5(2003):474-480.