The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins

	The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins
	Zhang, SB; Huang, J; Zhao, H; Zhang, Y; Hou, CH; Cheng, XD; Jiang, C; Li, MQ; Hu, J; Qian, RL
刊名	CELL RESEARCH
	2003
卷号	13 期号:5 页码:351-359
关键词	atomic force microscopy HMG proteins (HMG1/2 and HMG14/17) human beta-globin gene in vitro reconstitution nucleosome
通讯作者	Qian, RL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Grp Globin Gene Express &, Shanghai 200031, Peoples R China.,
英文摘要	Using atomic force microscopy (AFM), the dynamic process of the in vitro nucleosome reconstitution followed by slow dilution from high salt to low salt was visualized. Data showed that the histone octamers were dissociated from DNA at 1MNaCl. When the salt concentration was slowly reduced to 650 mM and 300 mM, the core histones bound to the naked DNA gradually. Once the salt concentration was reduced to 50 mM the classic "beads-on-a-string" structure was clearly visualized. Furthermore, using the technique of the in vitro reconstitution of nucleosome, the mono- and di- nucleosomes were assembled in vitro with both HS2core (- 10681 to - 10970 bp) and NCR2 (-3 72 to - 194 bp) DNA sequences in the 5'flanking sequence of human b-globin gene. Data revealed that HMG 1/2 and HMG14/17 proteins binding to both DNA sequences are changeable following the assembly and disassembly of nucleosomes. We suggest that the changeable binding patterns of HMG 14/17 and HMG1/2 proteins with these regulatory elements may be critical in the process of nucleosome assembly, recruitment of chromatin-modifying activities, and the regulation of human b-globin gene expression.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	BETA-GLOBIN GENE ; CIS-ACTING ELEMENTS ; CHROMOSOMAL-PROTEINS ; FORCE MICROSCOPY ; CORE PARTICLE ; DNA-SEQUENCES ; CHROMATIN ; HMG-17
收录类别	SCI
语种	英语
WOS记录号	WOS:000187509700006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2291]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, SB,Huang, J,Zhao, H,et al. The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins[J]. CELL RESEARCH,2003,13(5):351-359.
APA	Zhang, SB.,Huang, J.,Zhao, H.,Zhang, Y.,Hou, CH.,...&Qian, RL.(2003).The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins.CELL RESEARCH,13(5),351-359.
MLA	Zhang, SB,et al."The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins".CELL RESEARCH 13.5(2003):351-359.