Ectopic expression of the spike protein of Rice Ragged Stunt Oryzavirus in transgenic rice plants inhibits transmission of the virus to insects

	Ectopic expression of the spike protein of Rice Ragged Stunt Oryzavirus in transgenic rice plants inhibits transmission of the virus to insects
	Shao, CG; Wu, JH; Zhou, GY; Sun, G; Peng, BZ; Lei, JL; Jin, DD; Chen, SX; Upadhyaya, NM; Waterhouse, P
刊名	MOLECULAR BREEDING
	2003
卷号	11 期号:4 页码:295-301
关键词	RRSV transgenic rice vector transmission viral spike protein
通讯作者	Gong, ZX (reprint author), Acad Sinica, Shanghai Inst Life Sci, Inst Biochem & Cell Biol, Key Lab Proteom, 320 Yue Yang Rd, Shanghai 20003, Peoples R China.,
英文摘要	Rice ragged stunt oryzavirus (RRSV) replicates in both its insect vector, Nilaparvata lugens, and its plant host, rice, and has a complex multi-component particle bearing spikes on its outer surface. Transgenic rice lines expressing the 39 kDa spike protein showed good resistance to infection by RRSV. Furthermore, N. lugens fed on these plants prior to feeding on RRSV-infected plants were significantly protected against RRSV infection. The viral titre in insects initially fed on transgenic plants and then on RRSV-infected plants was inversely proportional to the levels of the 39 kDa protein expressed in the transgenic plants. This suggests that the 39 kDa protein interferes with the interaction between the intact virus particles and insect cell receptors and that the spike protein of RRSV contributes to vector specificity. This approach would probably be a more environment-friendly and sustainable method of virus control than by actual eradication of insect vectors.
学科主题	Agriculture; Plant Sciences; Genetics & Heredity
类目[WOS]	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
关键词[WOS]	GENES
收录类别	SCI
语种	英语
WOS记录号	WOS:000182444000006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2280]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Shao, CG,Wu, JH,Zhou, GY,et al. Ectopic expression of the spike protein of Rice Ragged Stunt Oryzavirus in transgenic rice plants inhibits transmission of the virus to insects[J]. MOLECULAR BREEDING,2003,11(4):295-301.
APA	Shao, CG.,Wu, JH.,Zhou, GY.,Sun, G.,Peng, BZ.,...&Gong, ZX.(2003).Ectopic expression of the spike protein of Rice Ragged Stunt Oryzavirus in transgenic rice plants inhibits transmission of the virus to insects.MOLECULAR BREEDING,11(4),295-301.
MLA	Shao, CG,et al."Ectopic expression of the spike protein of Rice Ragged Stunt Oryzavirus in transgenic rice plants inhibits transmission of the virus to insects".MOLECULAR BREEDING 11.4(2003):295-301.