Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources

	Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources
	Xie, JL; Zhang, L; Ye, Q; Zhou, QW; Xin, L; Du, P; Gan, RB
刊名	BIOTECHNOLOGY LETTERS
	2003
卷号	25 期号:2 页码:173-177
关键词	angiostatin dissolved O-2 glycerol feeding Pichia pastoris
通讯作者	Ye, Q (reprint author), E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China.,
英文摘要	A recombinant strain of Pichia pastoris with a phenotype of Mut(S) was used to produce angiostatin. Due to the low methanol consumption rate of this strain, both methanol and glycerol feedings, that produced oscillation in dissolved O-2 concentration, were used during the expression phase to improve cell growth and angiostatin expression. However, enhanced cell growth led to nitrogen limitation that suppressed further production of angiostatin, but addition of ammonia allowed angiostatin concentration to reach 108 mg l(-1) after an expression period of 96 h. The ratio of consumed glycerol to methanol of 1.5:1 (w/w) in the expression phase suggested that methanol played an important role in the metabolism of carbon sources.
学科主题	Biotechnology & Applied Microbiology
类目[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	FERMENTATION ; PROTEIN ; GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000180344400015
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2259]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xie, JL,Zhang, L,Ye, Q,et al. Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources[J]. BIOTECHNOLOGY LETTERS,2003,25(2):173-177.
APA	Xie, JL.,Zhang, L.,Ye, Q.,Zhou, QW.,Xin, L.,...&Gan, RB.(2003).Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources.BIOTECHNOLOGY LETTERS,25(2),173-177.
MLA	Xie, JL,et al."Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources".BIOTECHNOLOGY LETTERS 25.2(2003):173-177.