Expression and characterization of Kringle 1-4.5 domains of human plasminogen

	Expression and characterization of Kringle 1-4.5 domains of human plasminogen
	Zhou, QW; Xie, JL; Xin, L; Xu, R; Ye, Q; Li, ZP; Gan, RB
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2003
卷号	35 期号:2 页码:138-142
关键词	K1-4. 5 Pichia pastoris expression purification bioactivity
通讯作者	,
英文摘要	The cDNA encoding Kringle 1-4 and part of Kringle 5 domains of human plasminogen (K1-4.5), obtained from HepG2 by RT-PCR, was cloned into expression vector pHIL-SI. The recombinant plasmid pHIL-K 1-4. 5 was transformed into Pichia pastoris GS115 and the recombinant yeast was induced to express the recombinant proteins by methanol. The expressed proteins were purified by lysine affinity chromatography to a purity of 95%. The recombinant K 1-4. 5 inhibited the growth of bovine capillary endothelial cells ( BAEC) stimulated by the basic fibroblast growth factor ( bFGF), in a dosage-dependent manner with a half maximal concentration of 2 mg/L. rhKl-4.5 also inhibited 40% of the BAEC migration stimulated by bFGF in the concentration of I mg/L.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	PICHIA-PASTORIS ; HUMAN ANGIOSTATIN ; GROWTH-FACTOR ; ANGIOGENESIS ; INHIBITOR ; SUPPRESSION ; CANCER
收录类别	SCI
语种	英语
WOS记录号	WOS:000181245500007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2253]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, QW,Xie, JL,Xin, L,et al. Expression and characterization of Kringle 1-4.5 domains of human plasminogen[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(2):138-142.
APA	Zhou, QW.,Xie, JL.,Xin, L.,Xu, R.,Ye, Q.,...&Gan, RB.(2003).Expression and characterization of Kringle 1-4.5 domains of human plasminogen.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(2),138-142.
MLA	Zhou, QW,et al."Expression and characterization of Kringle 1-4.5 domains of human plasminogen".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.2(2003):138-142.