Candida albicans Cdc37 interacts with the Crk1 kinase and is required for Crk1 production

	Candida albicans Cdc37 interacts with the Crk1 kinase and is required for Crk1 production
	Ni, J; Gao, YK; Liu, HP; Chen, JY
刊名	FEBS LETTERS
	2004
卷号	561 期号:1 页码:223-230
关键词	CaCDC37 Crk1 chaperone Candida albicans
通讯作者	Chen, JY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jychen@sunm.shcnc.ac.cn
英文摘要	Crk1, a Cdc2-related protein kinase from the human pathogenic fungus Candida albicans, plays an important role in hyphal development and virulence. To address its regulatory mechanisms, we searched for Crk1 interacting proteins by two-hybrid screening. A CDC37 ortholog (CaCDC37) was cloned from the screening with the Crk1 kinase domain as the bait. The CaCdc37 interacted preferentially with the kinase domain of Crk1 (Crk1N) as shown by two-hybrid and immuno-precipitation experiments. CaCDC37 could complement a cdc37 thermosensitive mutant (cdc37-34) of Saccharomyces cerevisiae. Importantly, Crk1 protein was hardly detectable in the cdc37-34 mutant at restrictive temperature. However, upon expression of CaCdc37 in the cdc37 mutant, CrkI protein was detected even at restrictive temperature. Our data suggested that CaCdc37 was required for the production of Crk1 kinase. Like Cdc37 proteins of S. cerevisiae and higher eukaryotes, CaCdc37 might function as a molecular chaperone that stabilized Crk1 and other protein kinases in C. albicans. In support of this, CaSTI1 was identified from a two-hybrid screen with the full-length Crk1 as the bait. CaSti1 showed two-hybrid interactions with both Crk1 and the CaCdc37. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
学科主题	Biochemistry & Molecular Biology; Biophysics; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	CYCLIN-DEPENDENT KINASE ; RNA-POLYMERASE-II ; PROTEIN-KINASE ; IN-VIVO ; PHYSICAL INTERACTION ; CO-CHAPERONE ; P-TEFB ; HSP90 ; BINDING ; DOMAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000220244100040
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2198]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ni, J,Gao, YK,Liu, HP,et al. Candida albicans Cdc37 interacts with the Crk1 kinase and is required for Crk1 production[J]. FEBS LETTERS,2004,561(1):223-230.
APA	Ni, J,Gao, YK,Liu, HP,&Chen, JY.(2004).Candida albicans Cdc37 interacts with the Crk1 kinase and is required for Crk1 production.FEBS LETTERS,561(1),223-230.
MLA	Ni, J,et al."Candida albicans Cdc37 interacts with the Crk1 kinase and is required for Crk1 production".FEBS LETTERS 561.1(2004):223-230.