Multiple mechanisms for Wnt11-mediated repression of the canonical Wnt signaling pathway

	Multiple mechanisms for Wnt11-mediated repression of the canonical Wnt signaling pathway
	Maye, P; Zheng, J; Li, L; Wu, DQ
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2004
卷号	279 期号:23 页码:24659-24665
通讯作者	Wu, DQ (reprint author), Univ Connecticut, Ctr Hlth, Dept Genet & Dev Biol, MC3301,263 Farmington Ave, Farmington, CT 06030 USA.,dwu@neuron.uchc.edu
英文摘要	The effect of a noncanonical Wnt, Wnt11, on canonical Wnt signaling stimulated by Wnt1 and activated forms of LRP5 (low density lipoprotein receptor-related protein-5), Dishevelled1 (Dvl1), and beta-catenin was examined in NIH3T3 cells and P19 embryonic carcinoma cells. Wnt11 repressed Wnt1-mediated activation of LEF-1 reporter activity in both cell lines. However, Wnt11 was unable to inhibit canonical signaling activated by LRP5, Dvl1, or beta-catenin in NIH3T3 cells, although it could in P19 cells. In addition, Wnt11-mediated inhibition of canonical signaling in NIH3T3 cells is ligand-specific; Wnt11 could effectively repress canonical signaling activated by Wnt1, Wnt3, or Wnt3a but not by Wnt7a or Wnt7b. Co-culture experiments with NIH3T3 cells showed that the co-expression of Wnt11 with Wnt1 was not an essential requirement for the inhibition, suggesting receptor competition as a possible mechanism. Moreover, in both cell types, elevation of intracellular Ca2+ levels, which can result from Wnt11 treatment, led to the inhibition of canonical signaling. This result suggests that Wnt11 might not be able to signal in NIH3T3. Furthermore, P19 cells were found to express both endogenous canonical Wnts and Wnt11. Knockdown of Wnt11 expression using siRNA resulted in increased LEF-1 reporter activity, thus indicating that Wnt11-mediated suppression of canonical signaling exists in vivo.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	CONVERGENT EXTENSION MOVEMENTS ; WNT/BETA-CATENIN ; BETA-CATENIN ; FRIZZLED HOMOLOGS ; WINGLESS SIGNAL ; XENOPUS EMBRYOS ; CELL FATE ; DROSOPHILA ; PROTEINS ; FAMILY
收录类别	SCI
语种	英语
WOS记录号	WOS:000221702500096
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2180]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Maye, P,Zheng, J,Li, L,et al. Multiple mechanisms for Wnt11-mediated repression of the canonical Wnt signaling pathway[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2004,279(23):24659-24665.
APA	Maye, P,Zheng, J,Li, L,&Wu, DQ.(2004).Multiple mechanisms for Wnt11-mediated repression of the canonical Wnt signaling pathway.JOURNAL OF BIOLOGICAL CHEMISTRY,279(23),24659-24665.
MLA	Maye, P,et al."Multiple mechanisms for Wnt11-mediated repression of the canonical Wnt signaling pathway".JOURNAL OF BIOLOGICAL CHEMISTRY 279.23(2004):24659-24665.