Adenoviral transfer of human interleukin-10 gene in lethal pancreatitis

	Adenoviral transfer of human interleukin-10 gene in lethal pancreatitis
	Chen, ZQ; Tang, YQ; Zhang, Y; Jiang, ZH; Mao, EQ; Zou, WG; Lei, RQ; Han, TQ; Zhang, SD
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2004
卷号	10 期号:20 页码:3021-3025
通讯作者	Zhang, SD (reprint author), Shanghai Med Univ 2, Affiliated Ruijin Hosp, Dept Surg, Shanghai 200025, Peoples R China.,chenziq@hotmail.com
英文摘要	AIM: To evaluate the therapeutic effect of adenoviral-vector-delivered human interleukin-10 (hIL-10) gene on severe acute pancreatitis (SAP) rats. METHODS: Healthy Sprague-Dawley (SD) rats were intraperitoneally injected with adenoviral IL-10 gene (AdvhIL-10), empty vector (Adv0) or PBS solution. Blood, liver, pancreas and lung were harvested on the second day to examine hIL-10 level by ELISA and serum amylase by enzymatic assay. A SAP model was induced by retrograde injection of sodium taurocholate through pancreatic duct. SAP rats were then administered with AdvhIL-10, Adv0 and PBS solution by a single intraperitoneal injection 20 min after SAP induction. In addition to serum amylase assay, levels of hIL-10 and tumor necrosis factor-alpha (TNF-alpha) were detected by RT-PCR, ELISA and histological study. The mortality rate was studied and analyzed by Kaplan-Meier and log rank analysis. RESULTS: The levels of hIL-10 in the pancreas, liver and lung of healthy rats increased significantly after AdvhIL-10 injection (1.42 ng/g in liver, 0.91 ng/g in pancreas); while there was no significant change of hIL-10 in the other two control groups. The concentration of hIL-10 was increased significantly in the SAP rats after AdvhIL-10 injection (1.68 ng/g in liver, 1.12 ng/g in pancreas) compared to the other two SAP groups with blank vector or PBS treatment (P<0.05). The serum amylase levels remained normal in the AdvhIL-10 transfected healthy rats. However, the serum amylase level was significantly elevated in the other two control SAP rats. In contrast, serum amylase was down-regulated in the AdvhIL-10 treated SAP groups. The TNF-alpha expression in the AdvhIL-10 treated SAP rats was significantly lower compared to the other two control SAP groups. The pathohistological changes in the AdvhIL-10 treated group were better than those in the other two control groups. Furthermore, the mortality of the AdvhIL-10 treated group was significantly reduced compared to the other two control groups (P<0.05). CONCLUSION: Adenoviral hIL-10 gene can significantly attenuate the severity of SAP rats, and can be used in the treatment of acute inflammation process.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
收录类别	SCI
语种	英语
WOS记录号	WOS:000208098000021
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2176]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chen, ZQ,Tang, YQ,Zhang, Y,et al. Adenoviral transfer of human interleukin-10 gene in lethal pancreatitis[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(20):3021-3025.
APA	Chen, ZQ.,Tang, YQ.,Zhang, Y.,Jiang, ZH.,Mao, EQ.,...&Zhang, SD.(2004).Adenoviral transfer of human interleukin-10 gene in lethal pancreatitis.WORLD JOURNAL OF GASTROENTEROLOGY,10(20),3021-3025.
MLA	Chen, ZQ,et al."Adenoviral transfer of human interleukin-10 gene in lethal pancreatitis".WORLD JOURNAL OF GASTROENTEROLOGY 10.20(2004):3021-3025.