Insulin-like growth factor-1 receptor signaling in 3T3-L1 adipocyte differentiation requires lipid rafts but not caveolae

	Insulin-like growth factor-1 receptor signaling in 3T3-L1 adipocyte differentiation requires lipid rafts but not caveolae
	Hong, S; Huo, H; Xu, J; Liao, K
刊名	CELL DEATH AND DIFFERENTIATION
	2004
卷号	11 期号:7 页码:714-723
关键词	adipocyte differentiation IGF-1 receptor signaling : lipid rafts caveolae caveolins RNA interference 3T3-L1 cell
通讯作者	Liao, K (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn
英文摘要	Previously, we have found that lipid rafts/caveolae were essential for insulin-like growth factor-1 (IGF-1) receptor signaling during 3T3-L1 preadipocytes differentiation induction. However, it was not identified as to which of the membrane lipid-ordered microdomains mediates the receptor signal. Using small double-stranded RNA-mediated interference (RNAi), we successfully suppressed the caveolin-1 protein expression. In cells stably transfected with vector expressing small interfering RNA ( siRNA) fragment, no caveolin-1 protein or caveola was detected. On the other hand, removal of caveolin-1 did not affect the caveolinless lipid rafts or the localization of IGF-1 receptor in lipid rafts on plasma membrane. IGF-1 receptor signal transduction and induced cellular differentiation were normal in RNAi cells with only lipid rafts. Furthermore, these IGF-1 receptor signaling events were still sensitive to the cholesterol-binding reagents. Thus, our results suggest that lipid rafts are sufficient for IGF-1 receptor signaling and the recruitment of signal molecules by caveolin-1 is not essential for IGF-1 receptor signaling.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	PREADIPOCYTE DIFFERENTIATION ; PHOSPHATIDYLINOSITOL 3-KINASE ; CHOLESTEROL TRAFFICKING ; GENE-EXPRESSION ; PROTEIN ; CELLS ; MEMBRANE ; KINASE ; TRANSDUCTION ; MICRODOMAINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000222103800004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2109]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hong, S,Huo, H,Xu, J,et al. Insulin-like growth factor-1 receptor signaling in 3T3-L1 adipocyte differentiation requires lipid rafts but not caveolae[J]. CELL DEATH AND DIFFERENTIATION,2004,11(7):714-723.
APA	Hong, S,Huo, H,Xu, J,&Liao, K.(2004).Insulin-like growth factor-1 receptor signaling in 3T3-L1 adipocyte differentiation requires lipid rafts but not caveolae.CELL DEATH AND DIFFERENTIATION,11(7),714-723.
MLA	Hong, S,et al."Insulin-like growth factor-1 receptor signaling in 3T3-L1 adipocyte differentiation requires lipid rafts but not caveolae".CELL DEATH AND DIFFERENTIATION 11.7(2004):714-723.