Discovery of Ca2+-relevant and differentiation-associated genes downregulated in esophageal squamous cell carcinoma using cDNA microarray

	Discovery of Ca2+-relevant and differentiation-associated genes downregulated in esophageal squamous cell carcinoma using cDNA microarray
	Luo, AP; Kong, JP; Hu, GX; Liew, CC; Xiong, MM; Wang, XQ; Ji, JF; Wang, T; Zhi, HY; Wu, M
刊名	ONCOGENE
	2004
卷号	23 期号:6 页码:1291-1299
关键词	differentiation calcium cell envelope esophageal squamous cell carcinoma cDNA microarray
通讯作者	Liu, ZH (reprint author), Chinese Acad Med Sci, Inst Canc, Natl Lab Mol Oncol, Beijing 100021, Peoples R China.,liuzh@pubem.cicams.ac.cn
英文摘要	To identify genes that are differentially expressed in human esophageal squamous cell carcinoma (ESCC), we have developed a cDNA microarray representing 34 176 clones to analyse gene expression profiles in ESCC. A total of 77 genes ( including 31 novel genes) were downregulated, and 15 genes ( including one novel gene) were upregulated in cancer tissues compared with their normal counterparts. Immunohistochemistry and Northern blot analysis were carried out to verify the cDNA microarray results. It was revealed that genes involved in squamous cell differentiation were coordinately downregulated, including annexin I, small proline-rich proteins (SPRRs), calcium-binding S100 proteins (S100A8, S100A9), transglutaminase (TGM3), cytokeratins (KRT4, KRT13), gut-enriched Krupple-like factor ( GKLF) and cystatin A. Interestingly, most of the downregulated genes encoded Ca2+-binding or - modulating proteins that constitute the cell envelope (CE). Moreover, genes associated with invasion or proliferation were upregulated, including genes such as fibronectin, secreted protein acidic and rich in cystein ( SPARC), cathepsin B and KRT17. Functional analysis of the alteration in the expression of GKLF suggested that GKLF might be able to regulate the expression of SPRR1A, SPRR2A and KRT4 in ESCC. This study provides new insights into the role of squamous cell differentiation-associated genes in ESCC initiation and progression.
学科主题	Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity
类目[WOS]	Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]	PROLINE-RICH PROTEINS ; ANNEXIN-I ; TERMINAL DIFFERENTIATION ; EPIDERMAL-KERATINOCYTES ; TRANSCRIPTION FACTORS ; CORNIFIED ENVELOPE ; DEPENDENT MANNER ; BARRIER FUNCTION ; EXPRESSION ; CANCER
收录类别	SCI
语种	英语
WOS记录号	WOS:000188892200014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2104]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Luo, AP,Kong, JP,Hu, GX,et al. Discovery of Ca2+-relevant and differentiation-associated genes downregulated in esophageal squamous cell carcinoma using cDNA microarray[J]. ONCOGENE,2004,23(6):1291-1299.
APA	Luo, AP.,Kong, JP.,Hu, GX.,Liew, CC.,Xiong, MM.,...&Liu, ZH.(2004).Discovery of Ca2+-relevant and differentiation-associated genes downregulated in esophageal squamous cell carcinoma using cDNA microarray.ONCOGENE,23(6),1291-1299.
MLA	Luo, AP,et al."Discovery of Ca2+-relevant and differentiation-associated genes downregulated in esophageal squamous cell carcinoma using cDNA microarray".ONCOGENE 23.6(2004):1291-1299.