Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry

	Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry
	Li, C; Hong, Y; Tan, YX; Zhou, H; Ai, JH; Li, SJ; Zhang, L; Xia, QC; Wu, JR; Wang, HY
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2004
卷号	3 期号:4 页码:399-409
通讯作者	Wang, HY (reprint author), Eastern Hepatobiliary Surg Hosp, 225 Changhai Rd, Shanghai 200438, Peoples R China.,hywangk@online.sh.cn ; zr@sibs.ac.cn
英文摘要	Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. This study combined the LCM with isotope-coded affinity tag (ICAT) technology and two-dimensional liquid chromatography to investigate the qualitative and quantitative proteomes of hepatocellular carcinoma (HCC). The effects of three different histochemical stains on tissue sections have been compared, and toluidine blue stain was proved as the most suitable stain for LCM followed by proteomic analysis. The solubilized proteins from microdissected HCC and non-HCC hepatocytes were qualitatively and quantitatively analyzed with two-dimensional liquid chromatography tandem mass spectrometry (2D-LC-MS/MS) alone or coupled with cleavable ICAT labeling technology. A total of 644 proteins were qualitative identified, and 261 proteins were unambiguously quantitated. These results show that the clinical proteomic method using LCM coupled with ICAT and 2D-LC-MS/MS can carry out not only large-scale but also accurate qualitative and quantitative analysis.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	COMPLEX PROTEIN MIXTURES ; GEL-ELECTROPHORESIS ; CANCER BIOMARKERS ; PROSTATE-CANCER ; HUMAN HEPATOMA ; CELL-LINES ; IDENTIFICATION ; TISSUE ; TECHNOLOGY ; MARKERS
收录类别	SCI
语种	英语
WOS记录号	WOS:000220426900009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2079]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, C,Hong, Y,Tan, YX,et al. Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry[J]. MOLECULAR & CELLULAR PROTEOMICS,2004,3(4):399-409.
APA	Li, C.,Hong, Y.,Tan, YX.,Zhou, H.,Ai, JH.,...&Zeng, R.(2004).Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry.MOLECULAR & CELLULAR PROTEOMICS,3(4),399-409.
MLA	Li, C,et al."Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry".MOLECULAR & CELLULAR PROTEOMICS 3.4(2004):399-409.