Effects of Huangqi (Hex) on inducing cell differentiation and cell death in K562 and HEL cells

	Effects of Huangqi (Hex) on inducing cell differentiation and cell death in K562 and HEL cells
	Cheng, XD; Hou, CH; Zhang, XJ; Xie, HY; Zhou, WY; Yang, L; Zhang, SB; Qian, RL
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2004
卷号	36 期号:3 页码:211-217
关键词	cell death and differentiation Huangqi (Hex) human erythroleukemia cell lines
通讯作者	Qian, RL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,qianlab@sunm.scenc.ac.cn
英文摘要	Huangqi (Astragalus membranaceus), a traditional Chinese medicine, has been used to ameliorate side effects of cancer chemotherapy in China. However, little is known about its molecular mechanisms. Here we show that induction of K562 or HEL cells with 1.5 mg/ml of Huangqi (Hex) (Components extracted from Huangqi) for 3-5 d results in the expression of beta-globin gene in both cell lines and leads to terminal differentiation. Morevoer, the apoptosis in HEL cells can be induced by increasing concentration of Huangqi (Hex) to 4.5 mg/ml for 3-5 d. Upregulation of Apaf-1, caspse-3 and acetylcholinesterase (AChE) in HEL cells may play a crucial role in the process op apoptosis. The prospect of inducing expression of adult (beta) globin gene and apoptosis selectively in cancer cells is obviously attractive from a therapeutic point of view.
学科主题	Biochemistry & Molecular Biology ; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	ALZHEIMERS FIBRILS ; ACETYLCHOLINESTERASE ; ACTIVATION ; EXPRESSION ; APOPTOSIS ; CHOLINESTERASES ; MEGAKARYOCYTES ; TUMORIGENESIS ; DISEASE ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000221073900008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2057]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Cheng, XD,Hou, CH,Zhang, XJ,et al. Effects of Huangqi (Hex) on inducing cell differentiation and cell death in K562 and HEL cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(3):211-217.
APA	Cheng, XD.,Hou, CH.,Zhang, XJ.,Xie, HY.,Zhou, WY.,...&Qian, RL.(2004).Effects of Huangqi (Hex) on inducing cell differentiation and cell death in K562 and HEL cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(3),211-217.
MLA	Cheng, XD,et al."Effects of Huangqi (Hex) on inducing cell differentiation and cell death in K562 and HEL cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.3(2004):211-217.