Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis

	Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis
	Zeng, R; Ruan, HQ; Jiang, XS; Zhou, H; Shi, L; Zhang, L; Sheng, QH; Tu, Q; Xia, QC; Wu Jr
刊名	JOURNAL OF PROTEOME RESEARCH
	2004
卷号	3 期号:3 页码:549-555
关键词	coronavirus proteome mass spectrometry glycosylation phosphorylation
通讯作者	Zeng, R (reprint author), CAS, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn ; wujr@sibs.ac.cn
英文摘要	The proteomes of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and its infected Vero E6 cells were detected in the present study. The cytosol and nucleus fractions of virus infected cells as well as the crude virions were analyzed either by one-dimensional electrophoresis followed by ESI-MS/MS identification or by shotgun strategy with two-dimensional liquid chromatography-ESI-MS/MS. For the first time, all of the four predicted structural proteins of SARS-CoV were identified, including S (Spike), M (Membrane), N (Nucleocapsid), and E (Envolope) proteins. In addition, a novel phosphorylated site of M protein was observed. The combination of these gel-base and non-gel methods provides fast and complimentary approaches to SARS-CoV proteome and can be widely used in the analysis of other viruses.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	ACUTE RESPIRATORY SYNDROME ; MOUSE HEPATITIS-VIRUS ; ENVELOPE PROTEIN ; MEMBRANE-PROTEIN ; CELL-DIVISION ; HOST-CELL ; REPLICATION ; GENOME
收录类别	SCI
语种	英语
WOS记录号	WOS:000222075500023
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2055]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zeng, R,Ruan, HQ,Jiang, XS,et al. Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis[J]. JOURNAL OF PROTEOME RESEARCH,2004,3(3):549-555.
APA	Zeng, R.,Ruan, HQ.,Jiang, XS.,Zhou, H.,Shi, L.,...&Wu Jr.(2004).Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis.JOURNAL OF PROTEOME RESEARCH,3(3),549-555.
MLA	Zeng, R,et al."Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis".JOURNAL OF PROTEOME RESEARCH 3.3(2004):549-555.