A novel oncolytic adenovirus targeting to telomerase activity in tumor cells with potent

	A novel oncolytic adenovirus targeting to telomerase activity in tumor cells with potent
	Zou, WG; Luo, CX; Zhang, ZL; Liu, J; Gu, JF; Pei, ZF; Qian, C; Liu, XY
刊名	ONCOGENE
	2004
卷号	23 期号:2 页码:457-464
关键词	oncolytic adenovirus E1A gene hTERT promoter telomerase cancer
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn
英文摘要	Telomerase is a therapeutic target for cancer. Human telomerase reverse transcriptase (hTERT), the catalytic subunit of the telomerase, is transcriptionaly upregulated exclusively in about 90#37; of cancer cells. Previous studies have demonstrated that hTERT promoter can control the expression of exogenous genes to the telomerase-positive cancer cells, thus hTERT promoter is an excellent candidate for generating cancer-specific oncolytic adenovirus. In this study, we devised a novel oncolytic adenovirus (Ad.TERT) by replacing the normal E1A regulatory elements with hTERT promoter. Ad.TERT displays cancer-specific E1A expression, virus replication and cytolysis in in vitro experiments. In animal experiments, intratumoral administration of Ad.TERT demonstrates potent antitumoral efficacy at least in two xenograft models (Bcap37 and BEL7404). Ad.TERT was targeted by the telomerase activity in cancer cells and has potent antitumoral efficacy in vivo, and since telomerase activity is a wide-ranged tumor marker, Ad.TERT could be a powerful therapeutic agent for a variety of cancers.
学科主题	Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity
类目[WOS]	Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]	REPLICATION-COMPETENT ADENOVIRUS ; SUICIDE GENE-THERAPY ; EXPRESSING BACTERIAL NITROREDUCTASE ; REVERSE-TRANSCRIPTASE PROMOTER ; CATALYTIC SUBUNIT HTERT ; HUMAN CANCER-CELLS ; HUMAN BONE-MARROW ; PROSTATE-CANCER ; HEPATOCELLULAR-CARCINOMA ; BAX GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000188098300016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2047]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zou, WG,Luo, CX,Zhang, ZL,et al. A novel oncolytic adenovirus targeting to telomerase activity in tumor cells with potent[J]. ONCOGENE,2004,23(2):457-464.
APA	Zou, WG.,Luo, CX.,Zhang, ZL.,Liu, J.,Gu, JF.,...&Liu, XY.(2004).A novel oncolytic adenovirus targeting to telomerase activity in tumor cells with potent.ONCOGENE,23(2),457-464.
MLA	Zou, WG,et al."A novel oncolytic adenovirus targeting to telomerase activity in tumor cells with potent".ONCOGENE 23.2(2004):457-464.