Characterization of the 3a protein of SARS-associated coronavirus in infected Vero e6 cells and SARS patients

	Characterization of the 3a protein of SARS-associated coronavirus in infected Vero e6 cells and SARS patients
	Zeng, R; Yang, RF; Shi, MD; Jiang, MR; Xie, YH; Ruan, HQ; Jiang, XS; Shi, L; Zhou, H; Zhang, L
刊名	JOURNAL OF MOLECULAR BIOLOGY
	2004
卷号	341 期号:1 页码:271-279
关键词	SARS coronavirus 3a protein spike protein proteome
通讯作者	Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Res Ctr Proteome Anal, Key Lab Proteon, 320 Yue-Yang Rd, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn ; bingsun@sibs.ac.cn ; wujr@sibs.ac.cn
英文摘要	Proteomics was used to identify a protein encoded by ORF 3a in a SARS-associated coronavirus (SARS-CoV). Immuno-blotting revealed that interchain disulfide bonds might be formed between this protein and the spike protein. ELISA indicated that sera from SARS patients have significant positive reactions with synthesized peptides derived from the 3a protein. These results are concordant with that of a spike protein-derived peptide. A tendency exists for co-mutation between the 3a protein and the spike protein of SARS-CoV isolates, suggesting that the function of the 3a protein correlates with the spike protein. Taken together, the 3a protein might be tightly correlated to the spike protein in the SARS-CoV functions. The 3a protein may serve as a new clinical marker or drug target for SARS treatment. (C) 2004 Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	TRANSMISSIBLE GASTROENTERITIS VIRUS ; PORCINE RESPIRATORY CORONAVIRUS ; SPIKE PROTEIN ; MEMBRANE-FUSION ; SEQUENCE ; GENOME ; IDENTIFICATION ; REVEALS ; ORF
收录类别	SCI
语种	英语
WOS记录号	WOS:000222925100022
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2009]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zeng, R,Yang, RF,Shi, MD,et al. Characterization of the 3a protein of SARS-associated coronavirus in infected Vero e6 cells and SARS patients[J]. JOURNAL OF MOLECULAR BIOLOGY,2004,341(1):271-279.
APA	Zeng, R.,Yang, RF.,Shi, MD.,Jiang, MR.,Xie, YH.,...&Wu, JR.(2004).Characterization of the 3a protein of SARS-associated coronavirus in infected Vero e6 cells and SARS patients.JOURNAL OF MOLECULAR BIOLOGY,341(1),271-279.
MLA	Zeng, R,et al."Characterization of the 3a protein of SARS-associated coronavirus in infected Vero e6 cells and SARS patients".JOURNAL OF MOLECULAR BIOLOGY 341.1(2004):271-279.