In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS

	In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS
	Luo, HB; Ye, F; Sun, T; Yue, LD; Peng, SY; Chen, J; Li, GW; Du, Y; Xie, YH; Yang, YM
刊名	BIOPHYSICAL CHEMISTRY
	2004
卷号	112 期号:1 页码:15-25
关键词	SARS nucleocapsid protein SPR circular dichmism fluorescence protein folding and unfolding
通讯作者	Shen, X (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Drug Discovery & Design Ct, Shanghai 201203, Peoples R China.,xshen@mail.shcnc.ac.cn ; hljiang@mail.shcnc.ac.cn
英文摘要	The major biochemical and thermodynamic features of nucelocapsid protein of SARS coronavirus (SARS-NP) were characterized by use of non-denatured gel electrophoresis, size-exclusion chromatographic and surface plasmon resonance (SPR) techniques. The results showed that SARS_NP existed in vitro as oligomer, more probably dimer, as the basic functional unit. This protein shows its maximum conformational stability near pH 9.0, and it seems that its oligomer dissociation and protein unfolding occur simultaneously. Thermal-induced unfolding for SARS_NP was totally irreversible. Both the thermal and chemical denaturant-induced denaturation analyses showed that oligomeric SARS_NP unfolds and refolds through a two-state model, and the electrostatic interactions among the charge groups of SARS_NP made a significant contribution to its conformational stability. (C) 2004 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics; Chemistry
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Chemistry, Physical
关键词[WOS]	ACUTE RESPIRATORY SYNDROME ; MOUSE HEPATITIS-VIRUS ; ENVELOPE PROTEIN ; ESCHERICHIA-COLI ; P-PROTEIN ; CORONAVIRUS ; RNA ; STABILITY ; IDENTIFICATION ; PURIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000224852300003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2005]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Luo, HB,Ye, F,Sun, T,et al. In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS[J]. BIOPHYSICAL CHEMISTRY,2004,112(1):15-25.
APA	Luo, HB.,Ye, F.,Sun, T.,Yue, LD.,Peng, SY.,...&Jiang, HL.(2004).In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS.BIOPHYSICAL CHEMISTRY,112(1),15-25.
MLA	Luo, HB,et al."In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS".BIOPHYSICAL CHEMISTRY 112.1(2004):15-25.