Substitution of proline 306 in the reverse transcriptase domain of hepatitis B virus regulates replication

	Substitution of proline 306 in the reverse transcriptase domain of hepatitis B virus regulates replication
	Lin, X; Ma, ZM; Yao, X; He, LF; Yuan, ZH; Ding, JP; Wen, YM
刊名	JOURNAL OF GENERAL VIROLOGY
	2005
卷号	86 期号:1 页码:85-90
通讯作者	Wen, YM (reprint author), Fudan Univ, Shanghai Med Coll, Key Lab Med Mol Virol, 138 Yi Xue Yuan Rd, Shanghai 200032, Peoples R China.,ymwen@shmu.edu.cn
英文摘要	The proline residue at position 306 in hepatitis B virus (HBV) reverse transcriptase (rtP306) has been suggested to constrain the conformation of the a-helices in the thumb subdomain that interacts with the viral DNA template-primer. To study the impact of residue rt306 in HBV replication further, 11 site-directed mutants were constructed that substituted rtP306 with different amino acids. The replicative competencies of these mutants were assayed by HepG2 cell transfection and real-time PCR. When rtP306 was substituted with glycine or threonine, the replication competency of these mutants was drastically reduced to 1.96 and 4.51% of the wild-type HBV level, respectively. When rtP306 was substituted with glutamic acid, the replicative competency of the mutant increased up to 9.4-fold compared with wild-type virus, The results also showed that changes in the replicative competency of these constructed mutants were not associated with functional changes of HBV enhancer I. These results indicate the importance of amino acid(s) at the interface between the thumb and palm subdomains in modulating the replicative competency of HBV isolates. This regulatory residue(s) could serve as a new target for the development of anti-HBV drugs.
学科主题	Biotechnology & Applied Microbiology; Virology
类目[WOS]	Biotechnology & Applied Microbiology ; Virology
关键词[WOS]	DNA ; AMPLIFICATION ; TEMPLATE ; ELEMENTS ; REGION ; RNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000226390000010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2004]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Lin, X,Ma, ZM,Yao, X,et al. Substitution of proline 306 in the reverse transcriptase domain of hepatitis B virus regulates replication[J]. JOURNAL OF GENERAL VIROLOGY,2005,86(1):85-90.
APA	Lin, X.,Ma, ZM.,Yao, X.,He, LF.,Yuan, ZH.,...&Wen, YM.(2005).Substitution of proline 306 in the reverse transcriptase domain of hepatitis B virus regulates replication.JOURNAL OF GENERAL VIROLOGY,86(1),85-90.
MLA	Lin, X,et al."Substitution of proline 306 in the reverse transcriptase domain of hepatitis B virus regulates replication".JOURNAL OF GENERAL VIROLOGY 86.1(2005):85-90.