Separation, identification, and interaction of heparin oligosaccharides with granulocyte-colony stimulating factor using capillary electrophoresis and mass spectrometry

	Separation, identification, and interaction of heparin oligosaccharides with granulocyte-colony stimulating factor using capillary electrophoresis and mass spectrometry
	Liang, AY; Chao, YP; Liu, XJ; Du, YG; Wang, KY; Qian, SJ; Lin, BC
刊名	ELECTROPHORESIS
	2005
卷号	26 期号:18 页码:3460-3467
关键词	capillary electrophoresis granulocyte-colony stimulating factor heparin oligosaccharides
通讯作者	Lin, BC (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China.,bclin@dicp.ac.cn
英文摘要	A capillary electrophoresis (CE) method was developed for the separation of heparin oligosaccharides compatible to study the interactions between the oligosaccharides and granulocyte-colony stimulating factor (G-CSF). Unfractionated heparin was eliminitively degraded to heparin oligosaccharides by an endolytic heparinase. The degraded smaller oligosaccharides; (M-r < 1000) were baseline-separated by CE under a 50 mm phosphate buffer (pH 9.0) in 10 min. Standard heparin disaccharides and larger oligosaccharides (1000 < M-r < 8000) were all separated under optimized separation conditions. Compared with standard heparin disaccharides, smaller oligosaccharides contained one nonsulfated, two monosulfated, and two disulfated disaccharides, but trisulfated disaccharides were not found. The smaller oligosaccharides were also identified and molecular mass was deduced by electrospray ionization-mass spectrometry (ESI-MS). Furthermore, interactions between G-CSF and the oligosaccharides; were studied by using capillary zone electrophoresis (CZE) under the above separation conditions. It was found that larger oligosaccharides could interact with G-CSF while smaller oligosaccharides were not observed to bind to G-CSF under the experimental conditions. In conclusion, the purified heparinase could selectively degrade heparin into oligosaccharides and the interaction between G-CSF and heparin was correlated with the chain length of heparin.
学科主题	Biochemistry & Molecular Biology; Chemistry
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
关键词[WOS]	FIBROBLAST-GROWTH-FACTOR ; GLYCOSAMINOGLYCAN-DERIVED OLIGOSACCHARIDES ; CHONDROITIN SULFATE OLIGOSACCHARIDES ; INDUCED FLUORESCENCE DETECTION ; COMPOSITIONAL ANALYSIS ; ASSOCIATION CONSTANTS ; GEL-ELECTROPHORESIS ; PROTEIN INTERACTION ; BINDING SEQUENCE ; DISACCHARIDES
收录类别	SCI
语种	英语
WOS记录号	WOS:000232351800007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1983]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liang, AY,Chao, YP,Liu, XJ,et al. Separation, identification, and interaction of heparin oligosaccharides with granulocyte-colony stimulating factor using capillary electrophoresis and mass spectrometry[J]. ELECTROPHORESIS,2005,26(18):3460-3467.
APA	Liang, AY.,Chao, YP.,Liu, XJ.,Du, YG.,Wang, KY.,...&Lin, BC.(2005).Separation, identification, and interaction of heparin oligosaccharides with granulocyte-colony stimulating factor using capillary electrophoresis and mass spectrometry.ELECTROPHORESIS,26(18),3460-3467.
MLA	Liang, AY,et al."Separation, identification, and interaction of heparin oligosaccharides with granulocyte-colony stimulating factor using capillary electrophoresis and mass spectrometry".ELECTROPHORESIS 26.18(2005):3460-3467.