Derivation and growing human embryonic stem cells on feeders derived from themselves

	Derivation and growing human embryonic stem cells on feeders derived from themselves
	Wang, Q; Fang, ZF; Jin, F; Lu, Y; Gai, H; Sheng, HZ
刊名	STEM CELLS
	2005
卷号	23 期号:9 页码:1221-1227
关键词	human embryonic stem cells feeder
通讯作者	Sheng, HZ (reprint author), Shanghai Med Univ 2, Xinhua Hosp, Ctr Dev Biol, 1665 Kong Jiang Rd, Shanghai 200092, Peoples R China.,hzsheng@sh163.sta.net.cn
英文摘要	Human embryonic stem cells (hESCs) are pluripotent. They have the potential to differentiate into every cell type of an organism. Since many human somatic cell types have the ability to support the growth of hESCs, cells differentiated from hESCs may also be able to support the growth of themselves. We tested this hypothesis by growing hESCs on feeders derived from themselves and demonstrated that such feeders did constitute an environment suitable for the derivation and long-term growth of hESCs. hESCs maintained in this system expressed all the markers indicative of the undifferentiated state and gave rise to cell types representative of all three primary germ layers upon differentiation. By modifying the genome of hESCs, feeders with special features can be derived and mass produced. The system will facilitate large-scale production of hESCs in a standardized animal pathogen-free environment.
学科主题	Cell Biology; Biotechnology & Applied Microbiology; Oncology; Hematology
类目[WOS]	Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
关键词[WOS]	PROLONGED UNDIFFERENTIATED GROWTH ; LENTIVIRAL VECTORS ; PLURIPOTENCY ; LAYERS ; FIBROBLASTS ; MAINTENANCE ; EXPRESSION ; DISTINCT ; CULTURE ; NANOG
收录类别	SCI
语种	英语
WOS记录号	WOS:000232672700001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1949]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, Q,Fang, ZF,Jin, F,et al. Derivation and growing human embryonic stem cells on feeders derived from themselves[J]. STEM CELLS,2005,23(9):1221-1227.
APA	Wang, Q,Fang, ZF,Jin, F,Lu, Y,Gai, H,&Sheng, HZ.(2005).Derivation and growing human embryonic stem cells on feeders derived from themselves.STEM CELLS,23(9),1221-1227.
MLA	Wang, Q,et al."Derivation and growing human embryonic stem cells on feeders derived from themselves".STEM CELLS 23.9(2005):1221-1227.