Cellular transformation by the MSP58 oncogene is inhibited by its physical interaction with the PTEN tumor suppressor

	Cellular transformation by the MSP58 oncogene is inhibited by its physical interaction with the PTEN tumor suppressor
	Okumura, K; Zhao, MJ; DePinho, RA; Furnari, FB; Cavenee, WK
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2005
卷号	102 期号:8 页码:2703-2706
通讯作者	Cavenee, WK (reprint author), Univ Calif San Diego, Ludwig Inst Canc Res, San Diego Branch, La Jolla, CA 92093 USA.,wcavenee@ucsd.edu
英文摘要	The PTEN (phosphatase and tensin homologue) tumor suppressor protein contains a single catalytic domain with both lipid and protein phosphatase activities. The remaining C-terminal half of the PTEN protein plays a role in its stability and is mutated in many clinical cancer samples. Here, we report that the PTEN C-terminal domain physically interacts with the forkhead-associated domain of the oncogenic MSP58 protein and that this interaction requires PTEN Thr-366. We further show that while MSP58 transforms Pten-/- mouse embryo fibroblasts (MEFs), concurrent introduction of wild-type PTEN causes a dramatic reduction in the number of MSP58-induced transformed foci. This PTEN-mediated inhibition of cellular transformation requires physical interaction as evidenced by the failure of PTEN(T366A) point mutation (residing within the MSP58 interaction domain) to suppress MSP-58-driven transformation. These observations, together with the capacity of catalytically inactive PTEN mutant (G129R) to suppress MSP58 oncogenicity, support the view that the C-terminal region of PTEN directly provides a previously uncharacterized biological function in its ability to regulate cellular transformation.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	58-KDA MICROSPHERULE PROTEIN ; FHA DOMAIN ; PHOSPHORYLATION ; PHOSPHATASES ; MIGRATION ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000227232400009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1947]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Okumura, K,Zhao, MJ,DePinho, RA,et al. Cellular transformation by the MSP58 oncogene is inhibited by its physical interaction with the PTEN tumor suppressor[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2005,102(8):2703-2706.
APA	Okumura, K,Zhao, MJ,DePinho, RA,Furnari, FB,&Cavenee, WK.(2005).Cellular transformation by the MSP58 oncogene is inhibited by its physical interaction with the PTEN tumor suppressor.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,102(8),2703-2706.
MLA	Okumura, K,et al."Cellular transformation by the MSP58 oncogene is inhibited by its physical interaction with the PTEN tumor suppressor".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 102.8(2005):2703-2706.