Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches - Implications for cellular responses to virus infection

	Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches - Implications for cellular responses to virus infection
	Jiang, XS; Tang, LY; Dai, J; Zhou, H; Li, SJ; Xia, QC; Wu, JR; Zeng, R
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2005
卷号	4 期号:7 页码:902-913
通讯作者	Wu, JR (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Res Ctr Proteome Anal,Key Lab Proteom, 320 YueYang Rd, Shanghai 200031, Peoples R China.,wujr@sibs.ac.cn ; zr@sibs.ac.cn
英文摘要	We present the first proteomic analysis on the cellular response to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. The differential proteomes of Vero E6 cells with and without infection of the SARS-CoV were resolved and quantitated with two-dimensional differential gel electrophoresis followed by ESI-MS/MS identification. Moreover isotope-coded affinity tag technology coupled with two-dimensional LCMS/ MS were also applied to the differential proteins of infected cells. By combining these two complementary strategies, 355 unique proteins were identified and quantitated with 186 of them differentially expressed ( at least 1.5-fold quantitative alteration) between infected and uninfected Vero E6 cells. The implication for cellular responses to virus infection was analyzed in depth according to the proteomic results. Thus, the present work provides large scale protein-related information to investigate the mechanism of SARS-CoV infection and pathogenesis.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	MOUSE HEPATITIS-VIRUS ; NUCLEAR RIBONUCLEOPROTEIN A1 ; SARS-ASSOCIATED CORONAVIRUS ; DIFFERENTIAL GEL-ELECTROPHORESIS ; CODED AFFINITY TAGS ; 2-DIMENSIONAL ELECTROPHORESIS ; MURINE CORONAVIRUS ; MASS-SPECTROMETRY ; GENOME SEQUENCE ; VIRAL-RNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000230334500004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1938]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Jiang, XS,Tang, LY,Dai, J,et al. Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches - Implications for cellular responses to virus infection[J]. MOLECULAR & CELLULAR PROTEOMICS,2005,4(7):902-913.
APA	Jiang, XS.,Tang, LY.,Dai, J.,Zhou, H.,Li, SJ.,...&Zeng, R.(2005).Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches - Implications for cellular responses to virus infection.MOLECULAR & CELLULAR PROTEOMICS,4(7),902-913.
MLA	Jiang, XS,et al."Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches - Implications for cellular responses to virus infection".MOLECULAR & CELLULAR PROTEOMICS 4.7(2005):902-913.