Silk gland specific secretory expression of egfp gene in silkworm Bombyx mori with rAcMNPV system

	Silk gland specific secretory expression of egfp gene in silkworm Bombyx mori with rAcMNPV system
	Guo, XY; Guo, TQ; Wang, SP; Wang, JY; Lu, CD
刊名	ARCHIVES OF VIROLOGY
	2005
卷号	150 期号:6 页码:1151-1160
通讯作者	Lu, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,cdlu@sibs.ac.cn
英文摘要	To evaluate the possibility of establishing an in vivo baculovirus expression system in a silk gland specific secretory way, the recombinant Autographa californica nucleopolyhedrovirus (Acserpegfp Delta EGT) carrying the reporter gene egfp downstream of silkworm ser1 promoter and signal peptide coding sequence was generated. The purified recombinant baculovirus Acserpegfp Delta EGT was injected into the haemocoel of newly ecdysed 5(th) instar silkworm larvae at the amount of 10(6) pfu per larva. At 5 days post injection, green fluorescence derived from EGFP could be observed with fluorescent microscope in only the silk gland but not other tissues after dissection of the silkworm. By making an opening on the silk gland wall, green fluorescence could be observed in the outflow of silk gland indicating the secretion of EGFP and the effectiveness of ser1 signal peptide. Western blotting assay confirmed that EGFP exists in the water-soluble part of cocoon silk too. We also established a simple protocol to purify EGFP from the secreted silk proteins.
学科主题	Virology
类目[WOS]	Virology
关键词[WOS]	HIGH-LEVEL EXPRESSION ; BACULOVIRUS VECTOR ; INTERFERON ; PROTEIN ; INSECT ; LARVAE ; L.
收录类别	SCI
语种	英语
WOS记录号	WOS:000229240200008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1927]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Guo, XY,Guo, TQ,Wang, SP,et al. Silk gland specific secretory expression of egfp gene in silkworm Bombyx mori with rAcMNPV system[J]. ARCHIVES OF VIROLOGY,2005,150(6):1151-1160.
APA	Guo, XY,Guo, TQ,Wang, SP,Wang, JY,&Lu, CD.(2005).Silk gland specific secretory expression of egfp gene in silkworm Bombyx mori with rAcMNPV system.ARCHIVES OF VIROLOGY,150(6),1151-1160.
MLA	Guo, XY,et al."Silk gland specific secretory expression of egfp gene in silkworm Bombyx mori with rAcMNPV system".ARCHIVES OF VIROLOGY 150.6(2005):1151-1160.