A novel snoRNA can direct site-specific 2 '-O-ribose methylation of snRNAs in Oryza sativa

	A novel snoRNA can direct site-specific 2 '-O-ribose methylation of snRNAs in Oryza sativa
	Li, W; Jiang, G; Huang, B; Jin, YX
刊名	IUBMB LIFE
	2005
卷号	57 期号:3 页码:173-179
关键词	2 '-O-ribose methylation box C/D snoRNA snRNA Oryza sativa plant
通讯作者	Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,yxjin@sunm.shcnc.cn
英文摘要	Small nucleolar RNAs (snoRNAs) are a kind of noncoding RNAs, and the vast majority of snoRNAs are involved in site-specific modifications of rRNAs. A novel box C/D snoRNA called snoR124 was found in Oryza sativa, and it can direct 2'-O-ribose methylation of spliceosomal small nuclear RNAs (snRNAs). The snoRNA has two antisense elements, and the results of primer extensions at different dNTP concentrations provide evidence that snoR124 guide 2'-O-methylations of the C76 residue in the U4 snRNA and the T91 residue in the U5 snRNA. In addition, this snoRNA is located in a snoRNA gene cluster with another 7 snoRNAs which are identified to direct ribose methylations in rRNAs. This is consistent with the opinion that the snoRNA gene organization in plant is mainly gene cluster. The snoR124 is the first example of a snoRNA that directs modi. cations of RNAs other than rRNAs in plant; it will avail to get more insights into the function of snoRNAs in plant.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	SMALL NUCLEOLAR RNAS ; U6 SPLICEOSOMAL RNA ; SMALL NUCLEAR RNAS ; RIBOSOMAL-RNA ; PRERIBOSOMAL RNA ; MESSENGER-RNA ; U2 SNRNA ; PSEUDOURIDYLATION ; U5 ; 2'-O-METHYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000229006800006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1884]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, W,Jiang, G,Huang, B,et al. A novel snoRNA can direct site-specific 2 '-O-ribose methylation of snRNAs in Oryza sativa[J]. IUBMB LIFE,2005,57(3):173-179.
APA	Li, W,Jiang, G,Huang, B,&Jin, YX.(2005).A novel snoRNA can direct site-specific 2 '-O-ribose methylation of snRNAs in Oryza sativa.IUBMB LIFE,57(3),173-179.
MLA	Li, W,et al."A novel snoRNA can direct site-specific 2 '-O-ribose methylation of snRNAs in Oryza sativa".IUBMB LIFE 57.3(2005):173-179.