Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing

	Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing
	Cheng, GF; Lin, JJ; Feng, XG; Fu, ZQ; Jin, YM; Yuan, CX; Zhou, YC; Cai, YM
刊名	PROTEOMICS
	2005
卷号	5 期号:2 页码:511-521
关键词	differentially expressed proteins Schistosoma japonicum proteome pairing two-dimensional gel electrophoresis
通讯作者	Cai, YM (reprint author), Chinese Acad Agr Sci, Shanghai Inst Anim Parasitol, Key Lab Anim Parasitol, Minist Agr, 3,Lane 345,Shilong Rd, Shanghai 200232, Peoples R China.,caassp@public.sta.net.cn
英文摘要	Identification of differentially expressed proteins between the male and female worm of Schistosoma japonicum may provide new insights into the development of schistosomes, especially the molecular mechanism of female worm maturation induced by the male worm after pairing. Comparative two-dimensional gel electrophoresis (2-DE) and mass spectrometry were employed to separate and identify differentially expressed proteins between the male and female worm after pairing. Soluble and hydrophobic proteins from egg, schistosomulum (14 days), and female and male worms at adult stage (42 days) were separated by a sequential extraction method followed by 2-DE and 2-DE images were constructed. There were 1016 +/- 67, 1808 +/- 89, 1142 +/- 45 and 1288 +/- 32 spots detected for soluble proteins and 1425 +/- 108, 952 +/- 59, 847 +/- 75 and 965 +/- 69 spots for hydrophobic proteins from egg, schistosomulum, and adult stage female and male worms, respectively. The differentially and uniquely expressed proteins from male and female worms after pairing (42 days) include 41 +/- 4 and 23 +/- 2 unique spots for soluble proteins, and 11 +/- 1 and 26 +/- 3 unique spots for hydrophobic proteins, respectively. Matrix-assisted laser desorption/ionization-time of flight and electrospray ionization-tandem mass spectrometry were employed to analyze 12 unique spots from the female worm and 16 unique spots from the male worm for peptide mass fingerprinting and sequencing. The results showed that the main functions of these differentially expressed proteins were in signal transduction, metabolism and transcriptional regulation etc. Comparison of the schistosomes proteome between male and female worms may permit the identification of protein candidates for the development of vaccines or new targets for drug development against schistosomiasis.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology
关键词[WOS]	GENE-EXPRESSION ; MANSONI ; DNA ; STIMULATION ; GRADIENTS ; IMMATURE ; INVITRO ; GENOME
收录类别	SCI
语种	英语
WOS记录号	WOS:000227112100020
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1872]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Cheng, GF,Lin, JJ,Feng, XG,et al. Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing[J]. PROTEOMICS,2005,5(2):511-521.
APA	Cheng, GF.,Lin, JJ.,Feng, XG.,Fu, ZQ.,Jin, YM.,...&Cai, YM.(2005).Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing.PROTEOMICS,5(2),511-521.
MLA	Cheng, GF,et al."Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing".PROTEOMICS 5.2(2005):511-521.