Effects on human group X phospholipase A(2) inclusion bodies folding

	Effects on human group X phospholipase A(2) inclusion bodies folding
	Cheng, HQ; Zhang, C; Hu, HY; Xu, GJ; Cheng, HQ; Zhang, C; Hu, HY; Xu, GJ
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2005
卷号	32 期号:2 页码:154-160
关键词	human group X phospholipase A(2) inclusion body refolding L-arginine
通讯作者	Xu, GJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Grad Sch, Shanghai 200031, Peoples R China.,gjxu@sibs.ac.cn
英文摘要	Human group X phospholipase A(2) is a member of mammalian secretory phospholipase which belongs to phospholipase A(2) superfamily. It has been expressed in a form of inclusion bodies in E.coli. It could not retold efficiently as human pancreatic phospholipase A(2) (group I B) merely by diluting the protein unfolded in 8 mol/L of urea. The refolding reaction of human group X phospholipase A(2) in vitro was depended on temperature, pH and protein concentration. A number of additives have been tested, among which L-arginine was the most efficient effector in improving the refolding of human group X phospholipase A(2), and its structural analogs, L-citrulline, had less effect. L-lysine and L-arginine methyl ester could not improve phospholipase A(2) refolding. In addition, L-arginine inhibited the formation of aggregates and of intramolecular disulfide bonds. These results showed that both carboxyl and guanidyl residues of L-arginine were essential for improving protein refolding.
学科主题	Biochemistry & Molecular Biology ; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	ARACHIDONIC-ACID ; ESCHERICHIA-COLI ; MAMMALIAN-CELLS ; GROUP IB ; A(2) ; PROTEINS ; RELEASE ; AGGREGATION ; EXPRESSION ; ENZYMES
收录类别	SCI
语种	中文
WOS记录号	WOS:000227112400010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1871]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Cheng, HQ,Zhang, C,Hu, HY,et al. Effects on human group X phospholipase A(2) inclusion bodies folding[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2005,32(2):154-160.
APA	Cheng, HQ.,Zhang, C.,Hu, HY.,Xu, GJ.,Cheng, HQ.,...&Xu, GJ.(2005).Effects on human group X phospholipase A(2) inclusion bodies folding.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,32(2),154-160.
MLA	Cheng, HQ,et al."Effects on human group X phospholipase A(2) inclusion bodies folding".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 32.2(2005):154-160.