Histone hypoacetylation is involved in 1,10-phenanthroline-Cu2+-induced human hepatoma cell apoptosis

	Histone hypoacetylation is involved in 1,10-phenanthroline-Cu2+-induced human hepatoma cell apoptosis
	Kang, JH; Chen, J; Shi, YF; Jia, J; Wang, ZH
刊名	JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
	2005
卷号	10 期号:2 页码:190-198
关键词	reactive oxygen species histone hypoacetylation 1 histone acetyltransferase histone deacetylase 10-orthophenanthroline
通讯作者	Kang, JH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,kangjiuhong@lzu.edu.cn
英文摘要	The 1,10-orthophenanthroline (OP) - Cu2+ combination, one generally used reactive oxygen species (ROS) generation system, is known to induce cell apoptosis, but the mechanism of ROS generation in this process remains unclear. Here we found that in the presence of 5 mu M Cu2+, OP inhibited histone acetyltransferase ( HAT) activity, resulting in decreased acetylation in both histone H3 and H4. This inhibition of histone acetylation and HAT activity was significantly attenuated by preventing or scavenging ROS generation with the Cu2+ chelator of bathocuproine disulfonate, or the antioxidants of N-acetyl-cysteine and mannitol, respectively, indicating the involvement of ROS generation in OP - Cu2+ - induced histone hypoacetylation. At the same time, this ROS generation is found to be involved in OP - Cu2+ - induced apoptosis in human hepatoma Hep3B cells. The important role of histone hypoacetylation in the induction of apoptosis was also proven by the marked diminution of apoptosis by 100 nM trichostatin A, a specific inhibitor of histone deacetylase, or the overexpression of p300, an HAT protein. Collectively, these observations suggest that histone hypoacetylation represents one unrevealed mechanism involved in the in vivo function of OP - Cu2+- generated ROS, at least in their induction of cell apoptosis.
学科主题	Biochemistry & Molecular Biology; Chemistry
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
关键词[WOS]	GENE-EXPRESSION ; OXIDATIVE STRESS ; DEACETYLASE INHIBITORS ; H4 ACETYLATION ; DNA ; ACETYLTRANSFERASE ; DIFFERENTIATION ; COPPER-1,10-PHENANTHROLINE ; ACTIVATION ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000228258600011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1863]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Kang, JH,Chen, J,Shi, YF,et al. Histone hypoacetylation is involved in 1,10-phenanthroline-Cu2+-induced human hepatoma cell apoptosis[J]. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY,2005,10(2):190-198.
APA	Kang, JH,Chen, J,Shi, YF,Jia, J,&Wang, ZH.(2005).Histone hypoacetylation is involved in 1,10-phenanthroline-Cu2+-induced human hepatoma cell apoptosis.JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY,10(2),190-198.
MLA	Kang, JH,et al."Histone hypoacetylation is involved in 1,10-phenanthroline-Cu2+-induced human hepatoma cell apoptosis".JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY 10.2(2005):190-198.