Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells

	Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells
	Ma, R; Wu, SZ; Lin, QH
刊名	HORMONE RESEARCH
	2005
卷号	63 期号:1 页码:42169
关键词	androgen receptor testosterone auto-regulation of androgen receptor vascular smooth muscle cells androgen receptor transcriptional activity post-transcriptional regulation of androgen receptor
通讯作者	Wu, SZ (reprint author), Nanfang Hosp, Dept Cardiol, Guangzhou 510515, Peoples R China.,wusaizhu@fimmu.com
英文摘要	Objective: Androgens play an important role in the arterial vascular system, and androgen receptors (AR) have been identified in vascular smooth muscle cells (VSMCs). This study examined the effects of testosterone exposure on AR gene expression in cultured rat aortic smooth muscle cells. Methods: Changes in AR protein and messenger RNA ( mRNA) levels after androgen exposure were determined using immunoblotting and Northern blotting analysis respectively. Results: Treatment of synchronized VSMCs with testosterone increased both cytoplasmic and nuclear AR protein expression in a dose- and time-dependent fashion, whereas exposure of VSMCs to androgen for 10 min induced a transient down-regulation of AR protein. Meanwhile, AR mRNA level was also up-regulated, but to a much smaller extent. Pretreatment with transcription inhibitor and translation inhibitor repressed cytoplasmic AR protein levels to 46 and 12% ( means) of the androgen treatment control level respectively. Furthermore, androgen up-regulation of intracellular AR protein was partially inhibited by androgen antagonist. Conclusions: Androgen increases AR expression in VSMCs at the level of both transcription and non-transcription. Copyright (C) 2005 S. Karger AG, Basel.
学科主题	Endocrinology & Metabolism
类目[WOS]	Endocrinology & Metabolism
关键词[WOS]	CORONARY-HEART-DISEASE ; ANTIANDROGEN ACTION ; ESTROGEN-RECEPTOR ; DNA-SYNTHESIS ; SEX STEROIDS ; TESTOSTERONE ; ATHEROSCLEROSIS ; ARTERIES ; RELAXATION ; IMPAIRMENT
收录类别	SCI
语种	英语
WOS记录号	WOS:000226412400002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1847]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ma, R,Wu, SZ,Lin, QH. Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells[J]. HORMONE RESEARCH,2005,63(1):42169.
APA	Ma, R,Wu, SZ,&Lin, QH.(2005).Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells.HORMONE RESEARCH,63(1),42169.
MLA	Ma, R,et al."Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells".HORMONE RESEARCH 63.1(2005):42169.