Bistable expression of WOR1, a master regulator of white-opaque switching in Candida albicans

	Bistable expression of WOR1, a master regulator of white-opaque switching in Candida albicans
	Huang, GH; Wang, HF; Chou, S; Nie, XY; Chen, JY; Liu, HP
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2006
卷号	103 期号:34 页码:12813-12818
关键词	feedback regulation a1-alpha 2 repression
通讯作者	Chen, JY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jychen@sunm.shcnc.ac.cn ; h4liu@uci.edu
英文摘要	Candida albicans, a commensal organism and a pathogen of humans, can switch stochastically between a white phase and an opaque phase without an intermediate phase. The white and opaque phases have distinct cell shapes and gene expression programs. Once switched, each phase is stable for many cell divisions. White-opaque switching is under a1-alpha 2 repression and therefore only happens in a or a cells. Mechanisms that control the switching are unknown. Here, we identify Wor1 (white-opaque regulator 1) as a master regulator of white-opaque switching. The deletion of WOR1 blocks opaque cell formation. The ectopic expression of WOR1 converts all cells to stable opaque cells in a or a cells. In addition, the ectopic expression of WOR1 in a/alpha cells is sufficient to induce opaque cell formation. Importantly, WOR1 expression displays an all-or-none pattern. It is undetectable in white cells, and it is highly expressed in opaque cells. The ectopic expression of Wor1 induces the transcription of WOR1 from the WOR1 locus, which correlates with the switch to opaque phase. We present genetic evidence for feedback regulation of WOR1 transcription. The feedback regulation explains the bistable and stochastic nature of white-opaque switching.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	SACCHAROMYCES-CEREVISIAE ; POSITIVE FEEDBACK ; SCHIZOSACCHAROMYCES-POMBE ; HOMEODOMAIN PROTEINS ; FILAMENTOUS GROWTH ; MATING PHEROMONE ; GENE ; IDENTIFICATION ; TRANSITION ; PATHWAY
收录类别	SCI
语种	英语
WOS记录号	WOS:000240035900032
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1805]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Huang, GH,Wang, HF,Chou, S,et al. Bistable expression of WOR1, a master regulator of white-opaque switching in Candida albicans[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2006,103(34):12813-12818.
APA	Huang, GH,Wang, HF,Chou, S,Nie, XY,Chen, JY,&Liu, HP.(2006).Bistable expression of WOR1, a master regulator of white-opaque switching in Candida albicans.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,103(34),12813-12818.
MLA	Huang, GH,et al."Bistable expression of WOR1, a master regulator of white-opaque switching in Candida albicans".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 103.34(2006):12813-12818.