Transplantation of human amniotic epithelial cells improves hindlimb function in rats with spinal cord injury

	Transplantation of human amniotic epithelial cells improves hindlimb function in rats with spinal cord injury
	Wu, ZY; Hui, GZ; Lu, Y; Wu, X; Guo, LH
刊名	CHINESE MEDICAL JOURNAL
	2006
卷号	119 期号:24 页码:2101-2107
关键词	spinal cord injury human amniotic epithelial cells transplantation
通讯作者	Guo, LH (reprint author), Shanghai Celstar Inst Biotechnol, Shanghai 201203, Peoples R China.,lhguo@sibs.ac.cn
英文摘要	Background Human amniotic epithelial cells (HAECs), which have several characteristics similar to stem cells, therefore could possibly be used in cell therapy without creating legal or ethical problems. In this study, we transplanted HEACs into the injured spinal cord of rats to investigate if the cells can improve the rats' hindlimb motor function. Methods HAECs were obtained from a piece of fresh amnion, labeled with Hoechst33342, and transplanted into the site of complete midthoracic spinal transections in adult rats. The rats (n=21) were randomly divided into three groups: Sham-operation group (n=7), cells-graft group (n=7), and PBS group (n=7). One rat of each group was killed for histological analysis at the second week after the transplantation. The other six rats of each group were killed for histological analysis after an 8-week behavioral testing. Hindlimb motor function was assessed by using the open-field BBB scoring system. Survival rate of the graft cells was observed at second and eighth weeks after the transplantation. We also detected the myelin sheath fibers around the lesions and the size of the axotomized red nucleus. A one-way ANOVA was used to compare the means among the groups. The significance level was set at P < 0.05. Results The graft HAECs survived for a long time (8 weeks) and integrated into the host spinal cord without immune rejection. Compared with the control group, HAECs can promote the regeneration and sprouting of the axons, improve the hindlimb motor function of the rats (BBB score: cells-graft group 9.0 +/- 0.89 vs PBS group 3.7 +/- 1.03, P < 0.01), and inhibit the atrophy of axotomized red nucleus [cells-graft group (526.47 +/- 148.42) mu m(2) vs PBS group (473.69 +/- 164.73) mu m(2), P < 0.01]. Conclusion Transplantation of HAECs can improve the hindlimb motor function of rats with spinal cord injury.
学科主题	General & Internal Medicine
类目[WOS]	Medicine, General & Internal
关键词[WOS]	ADULT-RAT ; NEUROTROPHIC FACTORS ; RUBROSPINAL NEURONS ; AXONAL GROWTH ; REGENERATION ; EXPRESSION ; RECOVERY ; PROMOTE ; SURVIVAL ; TRACTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000243090400013
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1802]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wu, ZY,Hui, GZ,Lu, Y,et al. Transplantation of human amniotic epithelial cells improves hindlimb function in rats with spinal cord injury[J]. CHINESE MEDICAL JOURNAL,2006,119(24):2101-2107.
APA	Wu, ZY,Hui, GZ,Lu, Y,Wu, X,&Guo, LH.(2006).Transplantation of human amniotic epithelial cells improves hindlimb function in rats with spinal cord injury.CHINESE MEDICAL JOURNAL,119(24),2101-2107.
MLA	Wu, ZY,et al."Transplantation of human amniotic epithelial cells improves hindlimb function in rats with spinal cord injury".CHINESE MEDICAL JOURNAL 119.24(2006):2101-2107.