Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry

	Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry
	Li, DQ; Wang, L; Fei, F; Hou, YF; Luo, JM; Zeng, WCR; Wu, X; Lu, JS; Di, GH; Ou, ZL
刊名	PROTEOMICS
	2006
卷号	6 期号:11 页码:3352-3368
关键词	breast cancer mass spectrometry metastasis two-dimensional gel electrophoresis
通讯作者	Shao, ZM (reprint author), Fudan Univ, Canc Hosp, Inst Canc, Dept Breast Surg,Breast Canc Inst, 270 Dong An Rd, Shanghai 200032, Peoples R China.,zhimingshao@yahoo.com
英文摘要	To better understand the molecular mechanisms underlying breast cancer metastasis and search for potential markers for metastatic progression, we have developed a highly metastatic variant of human MDA-MB-435 breast cancer cell line through in vivo stepwise selection of pulmonary metastatic cells caused by parental MDA-MB-435 cells in the athymic mice. Comparative proteomic analysis using 2-DE and LC-IT-MS revealed that 102 protein spots were reproducibly altered more than three-fold between the selected variant and its parental counterpart. Eleven differentially expressed protein spots were identified with high confidence using SEQUEST with uninterpreted tandem mass raw data. Cathepsin D precursor, peroxiredoxin 6 (PDX6), heat shock protein 27 (HSP27), HSP60, tropomyosin 1 (TPM1), TPM2, TPM3, TPM4,14-3-3 protein epsilon, and tumor protein D54 were up-regulated in the highly metastatic variant, whereas alpha B-crystalline (CRAB) was only detected in its parental counterpart. Differential expression was confirmed for four proteins including PDX6, CRAB, TPM4, and HSP60 by real-time quantitative PCR and Western blotting analysis in our model. Immunohistochemical analysis in 80 breast cancer donors demonstrated a significant association of TPM4 (p = 0.002), HSP60 (p = 0.001), PDX6 (p = 0.002) but not CRAB (p = 0.113) staining with the presence of lymph node metastasis. In addition, TPM4 staining was also associated with clinical stage (p = 0.000), but no significant association was found between TPM4, PDX6, CRAB, and HSP60 expression and tumor size, hormone receptor, and HER-2 status (p > 0.05). The functional implication of these identified proteins was also discussed. These proteomic data are valuable and informative for understanding breast cancer metastasis and searching for potential markers for metastatic progression.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology
关键词[WOS]	ALPHA-B-CRYSTALLIN ; CARCINOMA-CELL-LINES ; GENE-EXPRESSION ; PROTEOMIC ANALYSIS ; ELECTROSPRAY IONIZATION ; CLINICAL-SIGNIFICANCE ; MDA-MB-435 CELLS ; MESSENGER-RNA ; CATHEPSIN-D ; TROPOMYOSIN-1
收录类别	SCI
语种	英语
WOS记录号	WOS:000238333400011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1777]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, DQ,Wang, L,Fei, F,et al. Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry[J]. PROTEOMICS,2006,6(11):3352-3368.
APA	Li, DQ.,Wang, L.,Fei, F.,Hou, YF.,Luo, JM.,...&Shao, ZM.(2006).Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry.PROTEOMICS,6(11),3352-3368.
MLA	Li, DQ,et al."Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry".PROTEOMICS 6.11(2006):3352-3368.