Optimized adaptor polymerase chain reaction method for efficient genomic walking

	Optimized adaptor polymerase chain reaction method for efficient genomic walking
	Xu, P; Hu, RY; Ding, XY
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2006
卷号	38 期号:8 页码:571-576
关键词	optimized adaptor PCR touchdown PCR genomic walking gene trap promoter
通讯作者	Ding, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol & Cell Biol, Shanghai 200031, Peoples R China.,xyding@sunm.shcnc.ac.cn
英文摘要	Genomic walking is one of the most useful approaches in genome-related research. Three kinds of PCR-based methods are available for this purpose. However, none of them has been generally applied because they are either insensitive or inefficient. Here we present an efficient PCR protocol, an optimized adaptor PCR method for genomic walking. Using a combination of a touchdown PCR program and a special adaptor, the optimized adaptor PCR protocol achieves high sensitivity with low background noise. By applying this protocol, the insertion sites of a gene trap mouse line and two gene promoters from the incompletely sequenced Xenopus laevis genome were successfully identified with high efficiency. The general application of this protocol in genomic walking was promising.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	LIGATION-MEDIATED PCR ; ASYMMETRIC INTERLACED PCR ; TOUCHDOWN PCR ; FLANKING DNA ; GENE ; AMPLIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000239784200008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1749]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, P,Hu, RY,Ding, XY. Optimized adaptor polymerase chain reaction method for efficient genomic walking[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(8):571-576.
APA	Xu, P,Hu, RY,&Ding, XY.(2006).Optimized adaptor polymerase chain reaction method for efficient genomic walking.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(8),571-576.
MLA	Xu, P,et al."Optimized adaptor polymerase chain reaction method for efficient genomic walking".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.8(2006):571-576.