Tyrosine residues of the extrinsic 23 kDa protein are important for its interaction with spinach PSII membranes

	Tyrosine residues of the extrinsic 23 kDa protein are important for its interaction with spinach PSII membranes
	Gao, JP; Zhang, F; Weng, J; Ruan, KC; Xu, CH
刊名	PROTEIN AND PEPTIDE LETTERS
	2006
卷号	13 期号:6 页码:539-544
关键词	chemical modification extrinsic 23 kDa protein circular dichroism reconstitution ability
通讯作者	Xu, CH (reprint author), Chinese Acad Sci, SIBS, Inst Plant Physiol, 345 Lingling Lu, Shanghai 200032, Peoples R China.,xch@iris.sipp.ac.cn
英文摘要	The 1, 4, and 8 tyrosine (Tyr) residues on the PSII extrinsic 23 kDa protein were modified with 5, 10 or 40 mM N-acetylimidazole (NAI) respectively. The amount of rebound NAI-modified extrinsic 23 kDa protein was 98%, 80%, and 5% of that in the unmodified protein, respectively. These results indicate that the Tyr residues are absolutely essential to reconstitution ability. Further, the fluorescence and circular dichroism spectra among native and NAI-modified extrinsic 23 kDa proteins were similar, suggesting that the modification by NAI did not markedly influence the basic secondary structure of the native conformation. Thus, we have concluded that the tyrosine residues in the extrinsic 23 kDa protein are important for interaction with PSII membranes. In addition, we found that the structure of the extrinsic 23 kDa protein is stable in suspension (pH 4-9 or T-m 25-55 degrees C).
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	OXYGEN-EVOLVING COMPLEX ; MANGANESE-STABILIZING PROTEIN ; PHOTOSYSTEM-II ; 33-KDA PROTEIN ; ELECTROSTATIC INTERACTION ; THERMAL INACTIVATION ; N-ACETYLIMIDAZOLE ; EVOLUTION ; CYANOBACTERIUM ; IDENTIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000238215700002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1735]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gao, JP,Zhang, F,Weng, J,et al. Tyrosine residues of the extrinsic 23 kDa protein are important for its interaction with spinach PSII membranes[J]. PROTEIN AND PEPTIDE LETTERS,2006,13(6):539-544.
APA	Gao, JP,Zhang, F,Weng, J,Ruan, KC,&Xu, CH.(2006).Tyrosine residues of the extrinsic 23 kDa protein are important for its interaction with spinach PSII membranes.PROTEIN AND PEPTIDE LETTERS,13(6),539-544.
MLA	Gao, JP,et al."Tyrosine residues of the extrinsic 23 kDa protein are important for its interaction with spinach PSII membranes".PROTEIN AND PEPTIDE LETTERS 13.6(2006):539-544.