Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus

	Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus
	Wang, ZQ; Han, YH; Shao, XX; Chi, CW; Guo, ZY
刊名	FEBS JOURNAL
	2007
卷号	274 期号:18 页码:4778-4787
关键词	conotoxin disulfide isomerization oxidative folding protein disulfide isomerase
通讯作者	Chi, CW (reprint author), Tongji Univ, Inst Prot Res, 1239 Siping Rd, Shanghai 200092, Peoples R China.,chi@sunm.shcnc.ac.cn ; zhan-yun.guo@mail.tongji.edu.cn
英文摘要	The oxidative folding of disulfide-rich conotoxins is essential for their biological functions. In vivo, disulfide bond formation is mainly catalyzed by protein disulfide isomerase. To elucidate the physiologic roles of protein disulfide isomerase in the folding of conotoxins, we have cloned a novel full-length protein disulfide isomerase from Conus marmoreus. Its ORF encodes a 500 amino acid protein that shares sequence homology with protein disulfide isomerases from other species, and 70% homology with human protein disulfide isomerase. Enzymatic analyses of recombinant C. marmoreus protein disulfide isomerase showed that it shared functional similarities with human protein disulfide isomerase. Using conotoxins tx3a and sTx3.1 as substrate, we analyzed the oxidase and isomerase activities of the C. marmoreus protein disulfide isomerase and found that it was much more efficient than glutathione in catalyzing oxidative folding and disulfide isomerization of conotoxins. We further demonstrated that macromolecular crowding had little effect on the protein disulfide isomerase-catalyzed oxidative folding and disulfide isomerization of conotoxins. On the basis of these data, we propose that the C. marmoreus protein disulfide isomerase plays a key role during in vivo folding of conotoxins.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	CONOTOXINS ; CATALYSIS ; SUPERFAMILY ; DEPENDENCE ; PEPTIDES ; HIRUDIN ; VENOMS ; DOMAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000249164800012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1627]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, ZQ,Han, YH,Shao, XX,et al. Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus[J]. FEBS JOURNAL,2007,274(18):4778-4787.
APA	Wang, ZQ,Han, YH,Shao, XX,Chi, CW,&Guo, ZY.(2007).Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus.FEBS JOURNAL,274(18),4778-4787.
MLA	Wang, ZQ,et al."Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus".FEBS JOURNAL 274.18(2007):4778-4787.