JMJD3 is a histone H3K27 demethylase

	JMJD3 is a histone H3K27 demethylase
	Xiang, Y; Zhu, ZQ; Han, G; Lin, HQ; Xu, LY; Chen, CD
刊名	CELL RESEARCH
	2007
卷号	17 期号:10 页码:850-857
关键词	histone methylation demethylase JMJD3 H3K27 prostate cancer
通讯作者	Chen, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,cdchen@sibs.ac.cn
英文摘要	Histone methylation is an important epigenetic phenomenon that participates in a diverse array of cellular processes and has been found to be associated with cancer. Recent identification of several histone demethylases has proved that histone methylation is a reversible process. Through a candidate approach, we have biochemically identified JMJD3 as an H3K27 demethylase. Transfection of JMJD3 into HeLa cells caused a specific reduction of trimethyl H3K27, but had no effect on di- and monomethyl H3K27, or histone lysine methylations on H3K4 and H3K9. The enzymatic activity requires the JmjC domain and the conserved histidine that has been suggested to be important for a cofactor binding. In vitro biochemical experiments demonstrated that JMJD3 directly catalyzes the demethylation. In addition, we found that JMJD3 is upregulated in prostate cancer, and its expression is higher in metastatic prostate cancer. Thus, we identified JMJD3 as a demethylase capable of removing the trimethyl group from histone H3 lysine 27 and upregulated in prostate cancer.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	DOMAIN-CONTAINING PROTEINS ; ANDROGEN-RECEPTOR ; PROSTATE-CANCER ; H3 LYSINE-9 ; METHYLATION ; FAMILY ; LSD1 ; TRANSCRIPTION ; EZH2
收录类别	SCI
语种	英语
WOS记录号	WOS:000251071100006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1602]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xiang, Y,Zhu, ZQ,Han, G,et al. JMJD3 is a histone H3K27 demethylase[J]. CELL RESEARCH,2007,17(10):850-857.
APA	Xiang, Y,Zhu, ZQ,Han, G,Lin, HQ,Xu, LY,&Chen, CD.(2007).JMJD3 is a histone H3K27 demethylase.CELL RESEARCH,17(10),850-857.
MLA	Xiang, Y,et al."JMJD3 is a histone H3K27 demethylase".CELL RESEARCH 17.10(2007):850-857.