Induction of a high population of neural stem cells with anterior neuroectoderm characters from epiblast-like P19 embryonic carcinoma cells

	Induction of a high population of neural stem cells with anterior neuroectoderm characters from epiblast-like P19 embryonic carcinoma cells
	Xia, CH; Wang, C; Zhang, KJ; Qian, C; Jing, NH
刊名	DIFFERENTIATION
	2007
卷号	75 期号:10 页码:912-927
关键词	epiblast neural stem cell P19 cell anterior neuroectoderm neural induction N2B27 BMP and FGF signaling
通讯作者	Jing, NH (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci,Lab Mol Cell Biol, Inst Biochem & Cell Biol,Key Lab Stem Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,njing@sibs.ac.cn
英文摘要	The epiblast, derived from the inner cell mass (ICM), represents the final embryonic founder cell population of mouse embryo and can give rise to all germ layer lineages including the neuroectoderm. The generation of neural stem cells from epiblast-like cells is of great value for studying the mechanism of neural determination during gastrulation stages of embryonic development. Mouse embryonic carcinoma (EC) P19 cells are equivalent to the epiblast of early post-implantation blastocysts. In this study, we establish a feasible induction system that allows rapid and efficient derivation of a high percentage (similar to 95%) of neural stem cells from P19 EC cell in N2B27 serum-free medium. The induced neural stem cells bear anterior neuroectoderm characters, and can be efficiently caudalized by retinoic acid (RA). These neural stem cells have multilineage potential to differentiate into neurons, astrocytes, and oligodendrocytes. Mechanistic analysis indicates that inhibition of the bone morphogenetic protein (BMP) pathway may be the main reason for N2B27-neural induction, and that fibroblast growth factor (FGF) signaling is also involved in this process. This method will provide an in vitro system to dissect the molecular mechanisms involved in neural induction of early mouse embryos.
学科主题	Cell Biology; Developmental Biology
类目[WOS]	Cell Biology ; Developmental Biology
关键词[WOS]	CENTRAL-NERVOUS-SYSTEM ; EARLY MOUSE EMBRYOS ; RETINOIC-ACID ; NEURONAL DIFFERENTIATION ; IN-VITRO ; HOMEOBOX GENE ; CHICK-EMBRYO ; RADIAL GLIA ; TRANSCRIPTION FACTOR ; XENOPUS EMBRYOS
收录类别	SCI
语种	英语
WOS记录号	WOS:000251385500003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1598]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xia, CH,Wang, C,Zhang, KJ,et al. Induction of a high population of neural stem cells with anterior neuroectoderm characters from epiblast-like P19 embryonic carcinoma cells[J]. DIFFERENTIATION,2007,75(10):912-927.
APA	Xia, CH,Wang, C,Zhang, KJ,Qian, C,&Jing, NH.(2007).Induction of a high population of neural stem cells with anterior neuroectoderm characters from epiblast-like P19 embryonic carcinoma cells.DIFFERENTIATION,75(10),912-927.
MLA	Xia, CH,et al."Induction of a high population of neural stem cells with anterior neuroectoderm characters from epiblast-like P19 embryonic carcinoma cells".DIFFERENTIATION 75.10(2007):912-927.